XBridge Columns by Waters

"Robust, easy to handle, good gradient transfer, maybe not big loading capacity"

"We have used the XBridge C18 columns for 10 years now and by taking good care, they still perform well. We saw that translation from analytical size to (semi-)prep works very well and continue to work with these columns."

"We ordered analytical column for our UPLC and preparative one for HPLC with the same chemistry in order to transfer directly our optimization in UPLC into the prep and it works fine. We use a column calculator for transferring the method."

"The XBridge prep column line from Waters continues their historical standard of high-end chromatography columns offered at an affordable price. This column has become the workhorse in our lab, and is used almost daily. I cannot recommend it highly enough."

"In our lab, we have been using it to purify 19 or 18 amino acid peptides. We have managed to purify different variants of the peptides. The resolution was very nice, and easy to manage, and can tolerate different buffer pH etc. Our oldest column is just about 1 year old. Unfortunately, for unknown reasons the yield we get was very low, and the resolution power went bad. We have been using it with utmost care, but this happened. We need to figure out what has happened."

"Generally this column is durable and provides high resolution to separate our chemicals. One thing we observed is the column still lost performance when we used it with acidic samples (ph~2)."

"Good column to use for separation. very Maximize Lab Efficiency. Improve Your LC I use several C18 HPLC columns, varying in column length and particle size, as well as packing material diameter, separate GMP starting materials, intermediates for API and drug products in standards and in various mother liquor. The column performances were compared both qualitatively and quantitatively. Factors investigated included efficiency, resolution, selectivity and analysis times, etc. Column lifetime was also examined. The results were used to evaluate the efficiency of the various columns for use in the chromatographic analysis. XBridge column is a very good column to use for separation, it let me complete method development and analytical support from starting materials to finished drug product."

"We have the Waters XBridge column on almost all of our preparative HPLC systems. Tran Pham is our representative and she has given us excellent customer service."

"The analytical and preparative XBridge columns are easy to use and produce reliable, replicable results. We chose this product after reviewing several columns in regard to material (C8/C18), length of the column, particle size and brand, and we are very happy with the product in general."

"These columns are a work-horse in our open access environment. We have found with regular flushing these column can last thousands of crude injections. I would highly recommend Waters BEH columns to other chromatographers."

"The column was a great addition to our laboratory, it works perfectly with the Preparative LC and the mass load is great. We can purify our target molecules and construct on a fast and reliable way from quite concentrated samples. Thanks to the method development tool from Waters, the method transfer from the Analytical scale to the prep scale allows us to keep a great resolution."

"Developed a UPLC method to isolate a minor impurity for characterization. Method transferred beautifully up to 50mM ID prep column with no issues."

"XBridge RP columns are very well suited for oligonucleotide reversed phase analysis and purification. Good resolution and life-time of columns."

"These columns provide us with excellent peak separation and reproducible elution times."

"Great RP Prep column. Full pH allows us to stock only this column for both Acidic and Basic."

"Unlike other vendors, the XBridge column is perfect for a wide range of pHs at minimal extra cost. So far we have injected >500 samples and have yet to see any deterioration in column performance. As coworkers have seen the performance of the XBridge column many are now relying more and more on this product in their day to day purifications. As a result we are expanding the number of columns we have to better suit their needs."

"We are using XBridge column for analytical and preparative chromatography. This is one of best columns for purification of small molecules. We quickly developed robust analytical methods using mobile phases throughout the entire pH range of 1-12 XBridge OBD™. Scale up to preparative."

"XBridge columns are most commonly used columns at our laboratory. We have C18 as well as C8 type. We have been offered a great discount. We could not work without these columns."

"Waters Application specialist help us to select the correct dimension of the column based on our application. On the receipt of the column, Application specialist was on site and help us to install and share his experience working with prep column. I am using this column almost every day and made more than 2000 injections with 10 mg/mL sample concentration and still, it works well for my application. I highly recommend this column and I am very happy with Waters products, their after sale support. The one thing that makes stand above all is their 90 days guarantee."

"The column can purify ~25 mg of peptides for each injection and great separation between the peaks. Great for purifying hydrophobic small peptides."

"We have been achieving high quality results (Peptides and nucleotides! Lots of charged species) and are very pleased with the quality of ALL Waters products!"

"The quality is good. But, the customer care after sales is really painful. For example, when I contacted them about a column that was bad right after we bought it, they made me go through day long trouble shooting steps without replacing, even though the column was used for the first time after buying."

"XBridge columns are easy to install and basic method development gives high resolution. They are robust, long-lasting and easily cleaned. Even when sample is highly concentrated, and material is overloaded, column resolution remains high."

"These are excellent columns. They come in a variety of sizes to meet every need, we use mostly the 19 x 100 mm. These columns are great and meet our routine purification needs and give excellent efficiency and resolution for those more challenging ones. We find that the columns are very robust, ours can last years and many injections, in some cases, a good flush is all that is needed to get them back to optimal. Highly recommended."

"The HPLC column provides sharp peaks in both weak acidic and basic conditions, and the retention times are quite consistent with extended periods of time. The column dies when sudden back-pressure increases, but normally after 3-6 months heavy use, probably 1500 injections. No special care is needed, as long as weak acid or base is used, but preferably maintaining at neutral conditions."

"We use three of the XBridge Prep C18 5μm OBD 19x150mm Columns in our company. The column is an effective device. We can get high quality products with reproducible results. It is very easy to install. Waters is a good partner with fast responses of our orders. Most of the time the orders are delivered on the next day via prime delivery."

"Column is easy to use and definitely reliable. It worked and separated my standard solutions nicely but it did not help me with separation of my reaction mixtures containing dsDNA of different length. E.g. I wanted to separate 31 bp oligonucleotide from 30 bp (or shorter) or 31 bp (or longer). The difference in molecular weight is too small that this column was not able to separate them. Even if I tried several and various gradients for separation."

"This product is 100% effective for our application, We can see the chemical purity of 18F-FDOPA (enantiomers and others reagents and impurities). In addition, it's so easy of use. The after-sales care is attentive and friendly. We think that the value of product is correct. Thanks for all."

"Most of the compounds can be separated well, load capacity is good. Reproducibility is good. over all I like this column."

"XBridge column shave proven to be superior quality compared to other options. They are compatible and reliable across different systems (both prep and analytical) and under different conditions."

"This reverse phase prep column proves to be a really worthy investment. Although it's a chromatography column, it is not at a consumable – in fact, it has lived longer than most of the other parts of the HPLC systems. It handles up to gram quantities of compounds with excellent separation power. We've had two for quite a few years, and the performance still holds up – no pressure buildup, no resident contamination on column. The elution profile is consistent over time, giving us excellent confidence in our compound purity."

"Good for specific resolution of polar compounds. We use them when other did not get to the separation of polar components of an active extract. Scale-ability for larger columns is less used here. Product is reliable and easy to use"

"Relatively easy to use for our basic analysis of ODN"

"This column is very good and gives very good separation."

"I used the column for Purification of some really difficult peptides. The results are very reproducible and I have been using it extensively for almost two years now, without any issues."

"That type of columns are very useful for our need. The columns are giving us good separation"

"Prep column worked very well and was the same separation as the analytical version."

"For the purpose of our application (natural products metabolites dereliction and isolation) the XBridge OBD prep column showed reproducible results from batch to batch runs as well as reliable comparison with the analytical run of the same sample so it is a very reliable and easy to use column."

"Column can achieve high quality and reproducible results. It is easy to use and lasts longer. Customer service is good if your ordering is wrong."

"We use an Xbridge C18 column for assaying raw material and actives in our Cosmetic products. It is a very useful column for method development because of its broad pH range and peak reproducibility. "

"I discovered this new HPLC column and it seems to be very promising for the separation of peaks (resolution). "

"The XBridge Amide column is prepared by high end capped silica particles, and it reduces or excludes our cation-exchange interaction when we use it in HILIC mode. HILIC phases are understood as mixed-mode phases, and the retention mechanism is difficult to understand due to its complex nature. In the case of XBridge Amide column, only hydrophilic partition mechanism seems to be important, and it is much easier to understand retention mechanism compared to another non-endo-capped stationary phases. In the case of a column, we do not need any after sales service, but we could find out the separation characteristics via the information from the company. The column is valuable in HILIC separation. It seems to be stable though the degree of hydrophilic nature and is somewhat lower than conventional amide bonded stationary phases. As mentioned above, this stationary phase is valuable to consider."

"Excellent columns for basic compounds separation and high stability. I used to compare with other stationary phases that are without hybrid silica and the results were poorer."

"The chromatogram is nice especially for the separation of smaller hydrophobic compounds."

"We used Xbridge columns for developing methods for assay and related substances. They are very robust and batch to batch reproducibility is excellent. With each column we do over 3000 injections."

"In my opinion XBridge columns are one of the best available on the market due to its universality. I am using it widely for analytical separations and (especially) for preparative purpose. The major advantages are: broad pH range; high stability at high pH; high durability and low column bleeding."

"The XBridge Waters columns are very easy to use. I used them in a comparison of different vendors columns. I must note that the after sales service did help me in my research."

"For ease of use, the chromatogram is fine and the column is reliable. My group like it. It is easily available and the work time is very long."

"I used XBridge Amide 3.5 um column for HILIC separation of atmospheric dicarboxylic acids. I found it very reproducible over a large period of time (> 3 months) while being extensively used during initial method development (use of different mobile phase buffers, pHs and organic solvents), method validation and application to atmospheric particulate matter. After more than 1000 injections with proper change of the guard, the analytical column preserved its initial efficiency and gave me the needed selectivity for separation of the various atmospheric acids. The column is still in good shape and operable. No increase of the column backpressure was observed during its use. We are very satisfied with the product and the level of service provided by Waters representatives."

"Great columns! Good resolution, fair price, good selection. Validation packs are helpful for method validation."

"This variety of column was a workhorse for a test that we were conducting; it was robust enough to keep running without much maintenance involved. The column we were using performed very well under the strict time constraints that we were working with."

"X-bridge columns: Waters very reliable columns for HPLC separations and work very well at a wide range of mobile phase pH conditions, and may be very suitable for pH optimization studies for method development. I would highly recommend these columns to a colleague.  If I was doing a pH optimization study, I would like to know if the column was good/stable over a wide pH range."

"Most of the time it uses formic acid buffer, but there are needs to use high pH buffer like 0.1 % NH4OH. The column performs well and is very stable. If using 2.5 µm particles, consider the XP format as it is cheaper according to the webpage."

"The columns are helpful in a wide variety of application areas. Products can withstand a wide pH range. They last and are fairly affordable. Have bought several and have yet to have a problem."

"Good, reliable, reproducible. Many methods were developed on this column with good result. It is good for basic conditions, have basic condition method with many injections, do not have column for 2 years and still running.  Good column, highly recommended to others. I like it very much, I will use it for basic conditions in the future."

"This column is used for our higher pressure methods and is very consistent. We will continue to use it for future products."

"This product is nice because it allows us to increase separation resolution without changing the validated method. The X-Bridge columns are part of Waters new smaller particle size columns. The chemistry of the stationary phase is the same as the 5um columns we have used in the past but packing is now 3.5um. We have just started using the columns so I cannot comment on the shelf life difference."

"This is a very rugged column in reversed phase chromatography with a wide pH range. It is an ideal column for method development with maximum flexibility across the entire pH range. This is a very good column for isolation and purification, the high pH stability of XBridge Prep columns allows for purer fractions and increased mass loading for bases. It gives batch to batch reproducibility, consistent results and extended lifetime under even the most demanding conditions. "

"BEH column separate in a lot of compounds with very nice sharp peaks."

"excellent performance."