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Description
A new milestone in chromatography
First introduced in 1999 in XTerra® columns, Waters patented organic/inorganic Hybrid Particle Technology [HPT] surmounted significant limitations of silica-based reversed-phase packing materials, particularly their hydrolytic instability at high pH. In 2005, second-generation HPT, branded as BEH Technology™, embodied in new ACQUITY UPLC™ BEH Columns and in Waters XBridge™ HPLC columns, marks a new milestone in chromatography.
With an order-of-magnitude improvement in high pH stability and a higher level of chromatographic performance, BEH Technology™ columns define the new benchmark for LC method development.
BEH Technology™, the second generation patented hybrid particle*, is a product of Waters commitment to continued investment in particle Research and Development, providing reliable solutions to complex method development problems. Based upon crucial customer feedback, three goals for this second-generation hybrid particle were considered paramount.
• Maximize efficiency
• Further improve high-pH stability
• Improve column reliability
BEH Technology™ hybrid particles are the succesful product of 4 years of research.
OBD™ Preparative Columns
Poor lifetimes and the lack of consistent performance of preparative columns have been a significant element of concern to the purification chemist. Cost per purification needs to be reduced and the loss of samples due to early column failure must be minimized. After many years of research into column packing and design, Waters introduced the patent pending Optimum Bed Density (OBD™) preparative column designs, effectively answering these concerns. This format is now recognized in the industry as delivering the most stable efficient and reproducible preparative columns.
The combination of the rugged XBridge™ packings and the OBD™ design takes preparative column performance to a new level, ensuring direct scalability, maximum efficiencies and the longest column lifetimes.
Application notes and news
- Comparison of Fully and Superficially Porous Particle Columns for the Analysis of Basic Compounds
- ACQUITY UPLC's Strong Performance is Moving the Entire Industry Forward
- New 2009 – 2010 Waters Chromatography Columns and Supplies Catalog Available
- Waters Introduces Two New Column Chemistries for Protein Separations
Reviews
Gregory Rahm Jr Status: See all reviews Member since 2013 Organization: Q Laboratories Read more » | RATING: 4.0 
"This variety of column was a workhorse for a test that we were conducting; it was robust enough to keep running without much maintenance involved. The column we were using performed very well under the strict time constraints that we were working with."   |
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| Vipan Taneja Status: See all reviews Member since 2013 Organization: Bristol Myers Squibb Read more » | RATING: 5.0
"X-bridge columns: Waters very reliable columns for HPLC separations and work very well at a wide range of mobile phase pH conditions, and may be very suitable for pH optimization studies for method development. I would highly recommend these columns to a colleague. If I was doing a pH optimization study, I would like to know if the column was good/stable over a wide pH range." |
| King Chan Status: See all reviews Member since 2010 Organization: SAIC-Frederick, Inc. Read more » | RATING: 5.0
"Most of the time it uses formic acid buffer, but there are needs to use high pH buffer like 0.1 % NH4OH. The column performs well and is very stable. If using 2.5 µm particles, consider the XP format as it is cheaper according to the webpage." |
| Mickey Rego Status: See all reviews Member since 2013 Organization: Averica Discovery Read more » | RATING: 4.3
"The columns are helpful in a wide variety of application areas. Products can withstand a wide pH range. They last and are fairly affordable. Have bought several and have yet to have a problem." |
| Sarah Chen Status: See all reviews Member since 2013 Organization: GlaxoSmithKline Read more » | RATING: 5.0
"Good, reliable, reproducible. Many methods were developed on this column with good result. It is good for basic conditions, have basic condition method with many injections, do not have column for 2 years and still running. Good column, highly recommended to others. I like it very much, I will use it for basic conditions in the future." |
| ANDREW PHELPS Status: See all reviews Member since 2013 Organization: METRICS, INC. Read more » | RATING: 4.3
"This column is used for our higher pressure methods and is very consistent. We will continue to use it for future products." |
| Carol Stein Status: See all reviews Member since 2011 Organization: Copperhead Chemical Co Read more » | RATING: 4.0
"This product is nice because it allows us to increase separation resolution without changing the validated method. The X-Bridge columns are part of Waters new smaller particle size columns. The chemistry of the stationary phase is the same as the 5um columns we have used in the past but packing is now 3.5um. We have just started using the columns so I cannot comment on the shelf life difference." |
| Kunwar Sanjeev Singh Status: See all reviews Member since 2010 Organization: Parabolic Drugs Ltd. Read more » | RATING: 4.7
"This is a very rugged column in reversed phase chromatography with a wide pH range. It is an ideal column for method development with maximum flexibility across the entire pH range. This is a very good column for isolation and purification, the high pH stability of XBridge Prep columns allows for purer fractions and increased mass loading for bases. It gives batch to batch reproducibility, consistent results and extended lifetime under even the most demanding conditions. " |
| Purificacion Fernandez Status: See all reviews Member since 2011 Organization: University of Santiago de Compostela Read more » | RATING: 4.3
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| Lin Ye Status: See all reviews Member since 2011 Organization: FDA Read more » | RATING: 4.3
"BEH column separate in a lot of compounds with very nice sharp peaks." |
| Adrel Status: See all reviews Member since 2007 Organization: Lavipharm Read more » | RATING: 4.6
"excellent performance." |
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