Fluorescence-based applications like qPCR, particularly those involving small volumes, benefit from the significantly improved reflection characteristics of the white wells. Specimens of low DNA conc...read more
Fluorescence-based applications like qPCR, particularly those involving small volumes, benefit from the significantly improved reflection characteristics of the white wells. Specimens of low DNA concentration, too, can be more easily detected, on average two cycles earlier. Compared to transparent wells, a 10-fold fluorescence level can be measured with white wells.
Thanks to maximum signal reflection, Ct values are obtained earlier. In addition, the white plates minimize adverse effects like "cross talk" that would increase well-to-well variability in transparent wells. A new, highly transparent, pressure-sensitive qPCR tape for maximum fluorescence transmission complements the product range. Due to the innovative adhesive, the tape has excellent sealing properties with minimised evaporation values and will not stick to hands or gloves during application. It doesn’t show distortion under thermal stress and has a functional sealing range from -70°C to 110°C. In white plates sealed with this tape, the reagent volume (SYBR Green) per well can be reduced from 25 µl to 15 µl without significant changes in sensitivity and stability, enabling a 40 % decrease in qPCR reagent costs.
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