The method provides a sensitivity of 1 - 10 pg of protein and yields fast, permanent, hardcopy results on Kodak X-Omat Blue Autoradiography Film or on the KODAK Image Station. Membranes can be stripped and reprobed if stored wet between uses.
Western Lightning PLUS is an enhanced luminol-based substrate for HRP-catalyzed detection. It is an economical choice that provides twice the sensitivity of standard reagents, and up to eight times the sensitivity of some brands. Furthermore, it offers the best signal-to-noise ratio, and generates good results on either PVDF or nitrocellulose membranes.
PerkinElmer guarantees the performance of Western Blot Chemiluminescence Reagent Plus for 12 months from ship date, and unopened reagent can be stored for at least eight weeks at room temperature. This extended shelf life means you can buy and use Western Blot Chemiluminescence Reagent Plus with confidence.