Veriti® thermal cycler by Thermo Fisher Scientific Invitrogen

Manufacturer's Response

Hi Kirston,

Thank you for your comments. You are correct that in your assessment of Veriti that when the block is programmed to do a sub-ambient (cold) temperature, the lid temperature defaults to 50C. This is true unless you set the heated lid temperature default setting to “off” where it will maintain “ambient temperature” (the temperature of your outside environment) for the entirety of your run. However, we don’t recommend you turn off the heated lid due to the possibility of evaporation when the block heats up. Let me clear up a few misconceptions underlying your comments:

1. The temperature of the lid does not significantly affect the temperature of the sample provided you are running within the specified volume range (10-80ul). It only serves to reduce evaporation in the case of hotter temperatures, or to reduce condensation at the top of the tube in the case of cooling. Our temperature accuracy specification for your sample is +/- 0.25C, which means that your sample will always be within 0.25C of the programmed set point, regardless of lid temperature.

2. We have done reliability testing with Veriti at 4C in adverse environmental conditions (high humidity, and ambient temperatures) for 72 hours. This means that you can confidently leave your samples in the machine on a Friday afternoon, and pick them up on Monday morning with no adverse effects. Using the instrument at a 4C hold on the instrument does not reduce the reliability of the instrument.

I hope that your Veriti continues to provide you quality PCR product for years to come. We are sure it will. Best of luck in your future experiments.