VECTASHIELD® Mounting Medium from Vector Laboratories is an aqueous mountant which does not solidify but remains a viscous liquid on the slide. To mount tissues or cells on a slide, a special pipet is supplied with the product to dispense one drop (approximately 25µl) onto the section. After a cover slip is applied, the VECTASHIELD® Mounting Medium is allowed to disperse over the entire section. (25µl is sufficient for a 22 x 22 mm coverslip). After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 ºC. The fluorescence will remain for many weeks.
Key features of VECTASHIELD Mounting Medium include:
• Prevents rapid loss of fluorescence during microscopic examination • Retains its anti-fading ability during long-term storage • Inhibits photobleaching of Fluorescein, Texas Red®, Rhodamine, AMCA, and other fluorochromes • Unique, stable formula superior to buffered glycerol, polyvinyl alcohol-based mounting solutions, or those containing commonly used anti-fading agents • Optically clear