V3 Western Workflow™

by Bio-Rad

4.2/5.0 (7 reviews)

Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins. The complete V3 workflow incorporates traditional blotting techniques with innovative tools, such as TGX Stain-Free™ precast gels, the Trans-Blot® Turbo™ system, and the ChemiDoc™ MP imager, to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection to obtain results faster.V3 Western Workflow™ 5 Step Workflow: Separate proteins — Mini-PROTEAN® TGX Stain-Free™ precast gels and Criterion™ TGX Stain-Free™ precast gels offer superior protei...Read more

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Reviews

7 Scientists have reviewed this product


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Average Rating: 4.2

Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date: 16 May 2014

  • Application Area: Protein Transfer For Analysis

  • V3 Western Workflow™

"Bio Rad westren transfer system is very easy to use, as per my experience it takes only 45 mins- 60 minutes for blot transfer. More over as the buffer tank is made as single unit without joints as compared with other companies, there is no tension of buffer leak from it. The electrophoresis blotting module is very easy to handle as it has got lock and key function. In nut shell advantages of western blot unit. 1. Its’ buffer tank 2. Blotting module 3. Least buffer required 4. Less time consuming 5. Easy handling "

Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:29 Nov 2013

  • V3 Western Workflow™

"This is a high end product with excellent reproducibility! I would recommend it if you are using western blotting. You should definitely purchase this product as it will ensure experimental excellence."
Rating: 3.3
Ease of use
3 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:29 Nov 2013

  • Application Area:Biochemical Analysis For Kinase Activation

  • V3 Western Workflow™

"It is a good, easy and quick way for western blot analysis. I wish it would be more sensitive for some low expression level protein detection. "
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:29 Oct 2013

  • Application Area:Signaling pathway studies

  • V3 Western Workflow™

"This system is very useful for gel documentation and analysis of protein expression using western blotting technique. This system gives good qualitative images of expressed proteins and quantitative analysis data. It is also a very good system for DNA and RNA analysis. This system is easy to use and analyze the data using software for JPEG, TIF and original images to generate the qualitative and quantitative data for publications."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:cancer biology

  • V3 Western Workflow™

"The products are easy to use. The antibodies are of good quality and give consistent results every time they are used."
Rating: 3.7
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:western blot

  • V3 Western Workflow™

"I think it is a very useful tool to determine the interested protein expression. I used this equipment in my research. My research is about the regulation of cell cycle. I got clear and significant results from it."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:cancer research

  • V3 Western Workflow™

"This product is easy to handle and sustainable. Results are clear and definite. Manuals are very easy to learn and follow even for new starters in the cancer biology. The system is not complicated therefore trouble shooting is always simple."

Description

Bio-Rad's V3 Western Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.

The complete V3 workflow incorporates traditional blotting techniques with innovative tools, such as TGX Stain-Free™ precast gels, the Trans-Blot® Turbo™ system, and the ChemiDoc™ MP imager, to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection to obtain results faster.


V3 Western Workflow™ 5 Step Workflow:

  1. Separate proteins — Mini-PROTEAN® TGX Stain-Free™ precast gels and Criterion™ TGX Stain-Free™ precast gels offer superior protein separation with fast run times and use standard Tris/glycine running buffers. TGX Stain-Free™ precast gels contain a unique technology formulated into the gel chemistry that enables rapid, high-quality separation of proteins in as little as 15 minutes.
  2. Visualize proteins — Protein separation can be visualized and confirmed using stain-free technology, which covalently binds proteins, after 1 minute of activation on a ChemiDoc MP imager. This allows the immediate visualization of proteins across the whole gel without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow.
  3. Transfer proteins — The Trans-Blot Turbo system is a rapid protein transfer apparatus that can reduce transfer protocols to as little as 3 minutes while maintaining high-efficiency protein transfers across a wide range of molecular weights.
  4. Verify protein transfer — The ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer.
  5. Validate and quantitate — The ChemiDoc MP imager and Image Lab™ software may be used to validate western blotting data via total protein normalization in conjunction with housekeeping proteins. By normalizing to total protein, stripping and reprobing is no longer necessary. Image Lab software gives quantitative results instead of relative normalization.

Product Overview

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V3 Western Workflow™

by Bio-Rad

4.2/5.0 (7 reviews)
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Receive your quote directly from Bio-Rad for V3 Western Workflow™