Agilents RNA Spike-In Kit allows researchers to efficiently monitor microarray workflow for linearity, sensitivity and accuracy. Each spike mix contains ten in vitro synthesized, polyadenylated transc...read more
Agilents RNA Spike-In Kit allows researchers to efficiently monitor microarray workflow for linearity, sensitivity and accuracy. Each spike mix contains ten in vitro synthesized, polyadenylated transcripts in predetermined ratios. When they are hybridized onto Agilent microarray control probes, the data needed to track performance and assure confidence is readily accessible.
Each transcript was constructed by the cloning of a unique 55-mer sequence into the human adenovirus type 6 E1A 13S gene
Sample required per reaction is 200 ng of Poly A(+) RNA, or 50 - > 2000 ng total RNA
Transcripts specifically anneal only to complementary microarray probes with minimal self- or cross-hybridization
Spike-In transcripts specifically anneal only to complementary control probes on Agilent's microarrays, with minimal self- or cross-hybridization
Kit should be stored initially at €“80°C for up to one year from date of result. Diluted spike mixes can be stored at €“20°C for up 2 months.
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on March 30th 2014).
Join the Global SelectScience Community Today! It’s FREE!
Helping you make informed decisions about the latest lab products and technologies:
Be the first to learn about new technologies
10,000+ trusted product reviews from your peers
Compare 500,000 products and manufacturers
Get exclusive buying tips from experts
Find solutions fast: 10,000+ Application Notes, Webinars and Videos
Connect with 250,000 scientists... All in one place!