Trypsin by Worthington Biochemical Corp.

For protein sequencing, the Worthington product Code: TRTPCK has been used successfully for many years. Worthington now offers two ‘Sequencing Grades’ which are purified and charactarized to meet the most stringent sequencing application requirements.

Likewise, for tissue culture work, Worthington trypsin has been used by many researchers through the years. We do not offer crude grades such as NF 1:250. Difficulties are often encountered when using these crude preparations including incomplete solubility, lot-to-lot variability, and cell toxicity. While purified trypsin alone may have limited effectiveness for tissue dissociation since it shows little reactivity with intercellular proteins, combinations of purified trypsin and other enzymes such as collagenase and elastase have proven effective for dissociation. The purified enzyme is especially useful for cell harvesting by a process called “trypsinization”.

For applications requiring particularly clean trypsin, Worthington offers VMF (Virus and Mycoplasma Free) Trypsin which is a specially processed trypsin. The product is irradiated to eliminate all organisms and is quality control tested specifically against Bovine Diarrhea virus, Infectious Bovine Rhinotracheitis (IBR) virus, Herpes virus, Enterovirus, Adenoviruses, Bovine syncytial virus, Parainfluenza type 3 (PI3-SF) and Parvovirus. Additionally, the material is tested to be free of mycoplasma. As a result of the purification process, extraneous proteases and nucleases which could be harmful to cell lines are removed.