TruSeq Custom Enrichment is an in-solution capture assay for targeting and enriching user-selected human genomic regions of ~500 kb - 25 Mb of cumulative DNA per sample for fully-customized resequencing. The assay is ideal for targeting specific genomic content, such as variant validation, examination of specific genes in pathways, or as a follow-up to GWAS.
Custom oligonucleotide probes are first designed and ordered in DesignStudio. The TruSeq Custom Oligo Set consists of 2,500 to 67,200 attempted custom oligos. TruSeq Custom Enrichment is fully integrated with TruSeq DNA Sample Preparation Kits for an optimized, gel-free workflow.
Based on the total number of samples requested, the TruSeq Custom Oligo Set includes sufficient 4-, 8- , 24-, or 96-reaction TruSeq Custom Enrichment reagent kits. Through pre-enrichment sample pooling of up to 12 samples, the 4-reaction kit supports 48 samples, the 8-reaction kit supports 96 samples, the 24-reaction kit supports 288 samples, and the 96-reaction kit supports 1,152 samples.