The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
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Trizma® hydrochloride
Average Rating: 4.0
1 Scientist has reviewed this product
Status:
Expert Reviewer
Member since: 2012
Organization: UNESP/IBILCE
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Rating: 4.0
Application Area: Making buffer solution
"Tris-HCl is a good buffer in the range 5.0-8.5. It is one of the best buffers used for crystallization of macromolecules. I recommend it to use as a buffer. "
Review date: 15 Dec 2014 | Trizma® hydrochloride
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
