Trisacryl® GF05 sorbent is composed of a highly hydrophilic copolymer designed for medium pressure gel filtration chromatography.
Trisacryl GF05 is designed for desalting and for the separation of small molecules.
Trisacryl GF05 provides a wide range of advantages over existing gels :
These characteristics allow Trisacryl GF05 sorbents to perform chromatographic separations quickly and with great selectivity under medium pressure, sterile conditions, and in the absence of any non-specific interactions with the matrix.
Chemical Composition and Structure
Trisacryl GF05 is a highly hydrophilic copolymer formed by the copolymerization of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol, and a hydroxylated acrylic bifunctional monomer.
The molecule features a high degree of hydrophilicity contributed by a secondary amide group, and by the presence of three primary hydroxymethyl groups per repeating unit.
Porosity
The modification of relative concentrations of the monomer and the cross-linking agent allow precise control of Trisacryl GF05 porosity. The exclusion limit of Trisacryl GF05 (determined using oligonucleotides) is 3,000 dt. Close control over the polymerization reaction guarantees not only a regular porosity inside each bead, but also excellent reproducibility from lot to lot.
Chemical Stability
Trisacryl GF05 is insoluble in chromatographic solvents commonly used in biochemistry (8 M urea, 6 M guanidine), as well as in detergents (Triton X-100, SDS, lubrol, ...). Trisacryl GF05 is resistant to acid treatments, making it an ideal matrix for peptide separations which require the use of acetic or hydrochloric acids. The matrix is not modified by incubation in the presence of 1 M HCl for several hours. However, Trisacryl GF05 is sensitive to strong alkaline agents such as sodium hydroxide. Sensitivity to alkaline medium is minimal in 0.1 M NaOH at +4 ºC for a maximum of one hour. If the NaOH concentration or the temperature are increased, the sorbent undergoes progressive hydrolysis of amide groups to give carboxyl groups. This will introduce some ion exchange properties.
Thermal Stability
Trisacryl GF05 is stable at high temperature (up to +121 ºC). It can be sterilized by autoclaving without undergoing any change in its chromatographic properties. However, the operation must be performed in buffered conditions, pH 7 in the absence of oxidizing agents.
Mechanical stability.
Due to its chemical composition which imparts rigidity, Trisacryl GF05 is resistant to pressures up to 2 - 3 bar. This exclusive property permits elevated flow rates. Resistance to micro-organisms. Due to its synthetic matrix, Trisacryl GF05 is resistant to microbial and enzymatic degradation.
Fractionation Range
The effective fractionation range for Trisacryl GF05 is between 200 and 2,500 dt. This was determined with globular proteins below 1000 kDa and extrapolates up to the exclusion limit.
Resolution Power
The resolution power of the M grade of Trisacryl GF05 (expressed by the number of theoretical plates per meter of column under normal running conditions) is relatively high — about 2,500 plates per meter or HETP (height equivalent to a theoretical plate) = 0.4 mm (value obtained using a 40 cm
column for a molecule eluted in the total available gel volume).
The resolution power of Trisacryl GF05 depends on the flow rate and varies with the molecular size of the product used. The flow rate needed to obtain optimal separation ranges from 2 to 3 cm/h. Because the height equivalent of a theoretical plate (HETP) increases very slowly as a function of flow rate (2 - 3 times less rapidly than with soft sorbents), Trisacryl GF05 provides excellent resolution power for proteins.
Manufacturer Pall Life Sciences Products - Biopharmaceutical Division
Be the first to review this product