TauTec’s TriMScope, a state-of-the-art multifocal multiphoton microscope, is based on a patented beam divider that splits up an incoming laser beam into up to 64 beamlets which are scanned simultaneously in the object plane. This results in either 64 times brighter images or 64 times higher image rates compared to standard single beam multiphoton scanning microscopes. The foci in the object plane are aligned in a single line and the number of foci can be easily switched from 64 to 32, 16, 8, 4 and to a single beam. Image rates up to 3600 Hz are possible. The TriMScope beam divider is a compact and easy-to-use device that utilizes exclusively flat optics for dividing the incoming beam with high light efficiency, avoiding aberrations and producing equally intense foci in the sample. As the beamlets are inherently displaced with respect to each other by several picoseconds, there is no cross-talk. Spatial and axial resolutions are diffraction limited. In addition, single line excitation allows convenient coupling to the input slit of an imaging spectrograph yielding real-time spectral sectioning in the x-y and x-z planes. TriMScope can be used to build up modular customized workshop stations or to upgrade an existing 2-photon microscope to a multi-technique microscope system for real time 3D fluorescence microscopy with time-lapse, ratio imaging, 2D and 3D kinetics, FLIM, FRET, FRAP, polarization and spectral functionalities.
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