Transcriptor First Strand cDNA Synthesis Kit


Roche Applied Science - a member of the Roche Group

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applicati...read more

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Description

 

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications:

  • Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments.
  • Generating cDNA libraries with large and full-length inserts.
  • Cloning genes of interest.

The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes 10 control reactions.

Benefits

Optimized for Many Applications

  • Use with conventional thermal cyclers and real-time PCR instruments.
  • Optimize real-time PCR – the kit is tested with the LightCycler® Carousel-Based System, the LightCycler® 480 System, and other real-time instruments.
  • Obtain accurate linear quantification over at least a 108-fold range of input RNA (in vitro transcripts), permitting the analysis of genes with very low or extremely high expression levels.
  • Generate efficient qPCR curves, with high fluorescence intensity and identical distances between RNA dilutions, allowing a straightforward analysis of results.
  • Obtain reliable and sensitive results – there are no additives in the RT buffer system that interfere with – or inhibit – the subsequent PCR.

Robustness

  • Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA with high secondary structures).
  • Achieve high reproducibility.
  • Generate full-length transcripts with the anchored-oligo(dT)18 primer that is included in the kit.
  • Obtain cDNA transcripts up to 14 kb.

Flexibility

  • Use three different priming methods – random hexamers, anchored-oligo(dT)18, and sequence-specific primers – depending on the type of analysis needed.

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