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Trail Mix™ Western Markers consist of the Perfect Protein™ Western Markers supplemented with a group of three prestained indicator proteins to allow direct visualization of protein mobility during electrophoresis. Trail Mix Western Markers and Western Blot Kits have all of the features of Perfect Protein Western Markers and Western Blot Kits, plus easy to track prestained bands at 15, 16 and 100 kDa. This mix also contains an additional Perfect Protein Western Marker at 225 kDa for improved sizing accuracy of very large proteins.
Features
• Trail Mix contains three prestained indicator proteins plus eight unstained Perfect Protein Western Markers.
• Perfect Protein Western Marker concentrations are optimized for blot detection.
• Each Perfect Protein Western Marker carries an S Tag™ and His Tag® fusion peptide.
• Kits contain S-protein AP or HRP Conjugate for convenient Western blot detection of markers.
• Conjugates can be added together with secondary antibody or streptavidin conjugates for simultaneous detection of target proteins and markers.
• Markers are supplied at the working dilution in gel loading buffer.
• Prestained proteins migrate at 15, 16 and 100 kDa. Proteins detected on Western blots are 15, 25, 35, 50, 75, 100, 150 and 225 kDa.
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