The Thermo Scientific Total RNA Isolation Reagent (TRIR) is designed for the extraction of total RNA from a variety of sources.
A biological sample is homogenised with the reagent using a glass-Teflon® homogeniser. After extraction with chloroform, the homogenate separates into two phases. RNA remains in the aqueous phase while DNA and proteins are extracted into the organic phase. The RNA is precipitated from the aqueous phase by addition of isopropanol, washed with ethanol and dissolved in RNase-free water.
The simplicity of the isolation using this procedure makes it possible to process a large number of samples simultaneously. The excellent recovery rate makes it ideal for the extraction of RNA from very small biological samples (biopsies, etc.).
Features • Extraction from most sample types • Consistent and reproducible • No degradation of RNA • Free of DNA and protein contamination • Total RNA in < 1 hour
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