TOPO® TA Cloning® Kit (with pCR®2.1-TOPO® Vector) with One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli by Thermo Fisher Scientific Invitrogen
Manufacturer Thermo Fisher Scientific Invitrogen
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants.pCR®-TOPO® Vectors include:3´-T overhangs for direct ligation of Taq-amplified PCR productsChoice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.EcoR I... Read more
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TOPO® TA Cloning® Kit (with pCR®2.1-TOPO® Vector) with One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli
Average Rating: 4.0
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Rating: 4.0
Review date: 27 Jan 2010 | TOPO® TA Cloning® Kit (with pCR®2.1-TOPO® Vector) with One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants.
pCR®-TOPO® Vectors include:
pCR®-TOPO® Vectors include:
- 3´-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
Product Overview
TOPO® TA Cloning® Kit (with pCR®2.1-TOPO® Vector) with One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli
Manufacturer Thermo Fisher Scientific Invitrogen
4.0 / 5.0 | 1 reviews