TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)

TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)

EMD Millipore, a division of Merck KGaA, Darmstadt, Germany

TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is ...read more

Request Pricing





Receive your quote directly from EMD Millipore, a division of Merck KGaA, Darmstadt, Germany for TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)

Description

 

TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum of 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653nm=3.9 x 104 mol-1 cm-1) and diimine (E450nm=5.9 x 104 mol-1 cm-1) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. ES022 is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunological assays.

Feedback

Request info

To:EMD Millipore, a division of Merck KGaA, Darmstadt, Germany
From:
Please contact me / call me with the TMB/E Ultra Sensitive, Blue, Horseradish Pero...,

I am looking to purchase in /

.
About you

Write a review

Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on May 30th 2014).

  
Your Email

 



Ease of use

After sales services

Value for money

WATCH:
The benefits of becoming a reviewer
Share your opinions...()
HINTS & TIPS
Give reasons for the star ratings you have awarded for each area.
Would you recommend this to a colleague?
What would you like to have known about equipment and consumables before you purchased?



Why make an enquiry with SelectScience?

  • EASY It’s quick and simple to do
  • FAST Your enquiry will be delivered straight to the manufacturer
  • FREE You’re under no obligation
  • SECURE We only pass your details on to trusted suppliers at your request
  • SAVE TIME Submit your details once and make multiple enquiries
SelectScience Trusted Banner