Effective image analysis in HCS cell-based assays and fluorescence microscopy requires fluorescent labeling of the entire cell. In these assays, the cellular primary object is used to identify and count individual cells and define the cell region in which the image analysis is applied. The primary object might be a major cellular component, such as the nucleus, a large organelle or the whole cell. When the whole cell is the primary object, high-quality Whole Cell Stains effectively distinguish intact cells from bordering cells when analyzed by appropriate software.
Manufacturer Thermo Fisher Scientific
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