Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits
 4.7/5.0 (1 review)

Thermo Fisher Scientific

The most popular amine-reactive biotinylation reagent for antibody labeling Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits Features: Water-soluble analog of NHS-LC-Biot...read more

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Reviews

Paul Gao


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Member since 2011

Organization:
Memorial Sloan-Kettering Cancer Center
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 08 Jan 2014

Application Area: Surface Protein Labelling
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits

"This product is great, reliable, economical and very easy to use, especially for the application of cell surface protein labelling. I have used it to test the membrane bound EGFR, and gp130 protein levels, and it has worked every time. The only drawback is this product has is that the company doesn't provide a small aliquoted packages, so it is a pain in the neck to measure up the small amounts every time, a bit annoying. But overall it is all good."

Description

 

The most popular amine-reactive biotinylation reagent for antibody labeling

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits Features:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond

Applications:

  • Enhanced detection of DNA on nitrocellulose
  • eliminating the need for radioactive detection in Southern
  • Northern, and dot blotting
  • Biotinylation of cell surface proteins
  • in vivo imaging and therapy studies

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