The Nucleofector Device

 3.5/5.0 (9 reviews)

Lonza Group Ltd

The Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector...read more

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Reviews

Marine Bretou


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Member since 2012

Organization:
Inst Curie
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 17 Dec 2012

Application Area: nucleoporation
The Nucleofector Device

"Our Nuclefector is used to transfect a wide range of cells, ranging from primary cells to cell lines. Nice efficiency for siRNA transfection into both primary cells and cell lines. On the other hand, if electroporation of DNA plasmids is efficient for cells lines, it is rather limited for primary cells (especially neurons and dendritic cells). A bit expensive, but really user friendly."

Read 9 reviews

Karim Ben M Barek


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Member since 2011

Organization:
Institut Curie
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RATING: 3.0

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 30 Nov 2011

The Nucleofector Device

"I use the Nuclefector Kit L and have found it to be simple and practical. It is the primary culture of rat cortical neurons. However it is expensive when you have to use for large scale."

Vidya Nadar


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Member since 2009

Organization:
Drexel Univ Coll Med
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 19 Jan 2011

The Nucleofector Device

"Great for neuronal transfections"

Timothy Angelotti


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Member since 2010

Organization:
Standford University
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RATING: 1.6

  • 2 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 1 out of 5VALUE FOR MONEY




Review date: 07 Dec 2010

The Nucleofector Device

"Very difficult to optimize for your cell types. All assistance is "classified"- Unable to discuss with TS & D staff."

Matt Larouche


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Member since 2010

Organization:
University of British Columbia
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RATING: 3.3

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 30 Nov 2010

The Nucleofector Device

"Good results with primary neurons. However, very difficult to troubleshoot because it is a 'Black Box'."

Koch Jonas


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Member since 2009

Organization:
IME Fraunhoffer
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RATING: 2.3

  • 2 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 19 Oct 2009

The Nucleofector Device

"The product gives fast and good results."

Deepika Vuppalanchi


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Member since 2007

Organization:
Univ Delaware
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 10 Jan 2008

The Nucleofector Device

Sujoy Dhara


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Member since 2007

Organization:
University Georgia
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RATING: 3.0

  • 3 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 09 Jan 2008

The Nucleofector Device

Becky Metcalf


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Member since 2006

Organization:
University of Nottingham
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 21 Sep 2006

The Nucleofector Device

"The equipment is easy to use and there is good technical support available."

Description

 

The Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions.

The electrical parameters of the Nucleofector are different from any other commercially available electroporation device. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type. As the electrical settings are pre-programmed into the Nucleofector, optimization of the electrical parameters by the user is not necessary.

The device weighs only 2.6 kg and is conveniently sized so that it easily fits under a sterile hood, which greatly facilitates sterile work with cells.

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