The Nucleofector Device by Lonza Group Ltd

Manufacturer Lonza Group Ltd
3.6
/
5.0
  |  11 reviews


The Nucleofector Device by Lonza Group Ltd product image
The Nucleofector Device
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Average Rating: 3.6
11 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money


Needs improvement
Rating: 3.0

  • Application Area: Electroporation of neuronal primary cultures

"The Nucleofector is very easy to use but give quite low levels of expression of your construct of interest, at least in neuronal rat primary cultures. Moreover, a very high number of neurons are needed due to the high mortality of the electroporation procedure. In addition, you must use the manufacturer solutions for the procedure, with homemade solutions mortality increases and efficacy decreases. I know there is a new version of the instrument now available and they may ensure better results."

Review date: 15 May 2017 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: CWRU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Can't imagine doing transfection work without this nucleofector
Rating: 4.7

  • Application Area:Nucleofection -transfection technology

"This device is wonderful and gives very good results with ES and iPS cells. The equipment is east to use and technical support from the company is fantastic."

Review date: 04 Nov 2016 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: Inst Curie



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:nucleoporation

"Our Nuclefector is used to transfect a wide range of cells, ranging from primary cells to cell lines. Nice efficiency for siRNA transfection into both primary cells and cell lines. On the other hand, if electroporation of DNA plasmids is efficient for cells lines, it is rather limited for primary cells (especially neurons and dendritic cells). A bit expensive, but really user friendly."

Review date: 17 Dec 2012 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Institut Curie



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    2 out of 5
Rating: 3.0
"I use the Nuclefector Kit L and have found it to be simple and practical. It is the primary culture of rat cortical neurons. However it is expensive when you have to use for large scale."

Review date: 30 Nov 2011 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: Drexel Univ Coll Med



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Great for neuronal transfections"

Review date: 19 Jan 2011 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Standford University



  • Ease of use
    2 out of 5
    After sales service
    2 out of 5
    Value for money
    1 out of 5
Rating: 1.7
"Very difficult to optimize for your cell types. All assistance is "classified"- Unable to discuss with TS & D staff."

Review date: 07 Dec 2010 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: University of British Columbia



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.3
"Good results with primary neurons. However, very difficult to troubleshoot because it is a 'Black Box'."

Review date: 30 Nov 2010 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: IME Fraunhoffer



  • Ease of use
    2 out of 5
    After sales service
    2 out of 5
    Value for money
    3 out of 5
Rating: 2.3
"The product gives fast and good results."

Review date: 19 Oct 2009 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Univ Delaware



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

Review date: 10 Jan 2008 | The Nucleofector Device
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: University Georgia



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.0

Review date: 09 Jan 2008 | The Nucleofector Device


  • Status:

    Reviewer

  • Member since: 2006

  • Organization: University of Nottingham



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"The equipment is easy to use and there is good technical support available."

Review date: 21 Sep 2006 | The Nucleofector Device
The Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions.

The electrical parameters of the Nucleofector are different from any other commercially available electroporation device. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type. As the electrical settings are pre-programmed into the Nucleofector, optimization of the electrical parameters by the user is not necessary.

The device weighs only 2.6 kg and is conveniently sized so that it easily fits under a sterile hood, which greatly facilitates sterile work with cells.