TA Cloning® Kit (with pCR®2.1 Vector) without Chemically Competent E. coli by Thermo Fisher Scientific Invitrogen
Manufacturer Thermo Fisher Scientific Invitrogen
TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts.Some of the convenient features of the pCR® vectors are:3´-T overhangs for direct ligation of Taq-amplified PCR productsChoice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencingVersatile polylinker with flanking EcoR I... Read more
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TA Cloning® Kit (with pCR®2.1 Vector) without Chemically Competent E. coli
TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts.
Some of the convenient features of the pCR® vectors are:
Some of the convenient features of the pCR® vectors are:
- 3´-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- Choice of kanamycin or ampicillin selection
- Easy blue/white colony screening
- M13 forward and reverse primer sites for sequencing
Product Overview
TA Cloning® Kit (with pCR®2.1 Vector) without Chemically Competent E. coli
Manufacturer Thermo Fisher Scientific Invitrogen
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