TA Cloning® Kit (with pCR® II vector) with One Shot® TOP10F´ Chemically Competent E. coli by Thermo Fisher Scientific Invitrogen
TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts.Some of the convenient features of the pCR® vectors are:3´-T overhangs for direct ligation of Taq-amplified PCR productsChoice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencingVersatile polylinker with flanking EcoR I...
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