T4 DNA Polymerase

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid.

Applications:
3´ overhang removal to form blunt ends (1,2).
5´overhang fill-in to form blunt ends (1,2).
Single strand deletion subcloning (3).
Second strand synthesis in site-directed mutagenesis (4).
Probe labeling using replacement synthesis (1,2).

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Reviews

5 Scientists have reviewed this product


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Average Rating: 3.9

Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date: 07 Oct 2013

  • Application Area: DNA purification

  • T4 DNA Polymerase

"It is easy to use, the protocol is simple and quick. DNA purification can be used in a wide area of research, could led to finding potential protein that is coded from DNA."

Rating: 3.7
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
3 out of 5
  • Review date:06 Aug 2013

  • Application Area:leukemia genetics

  • T4 DNA Polymerase

"I use this for sub cloning PCR product. It's always reliable and powerful enough to get enough yield. Efficiency seems more time-dependent, sometimes I get low yield when I incubate just for a short time < 1hr."
Rating: 4.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:DNA cloning

  • T4 DNA Polymerase

"Excellent consistency and long term quality. This product works well with NEB's other products - I would recommend their entire line of enzymes to my colleagues."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:ligation

  • T4 DNA Polymerase

"Excellent product and great website. Company provides great service. Works well. Good value for money. Highly recommended. I have been using it for a while and it works every time."

Description

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid.

Applications:
3´ overhang removal to form blunt ends (1,2).
5´overhang fill-in to form blunt ends (1,2).
Single strand deletion subcloning (3).
Second strand synthesis in site-directed mutagenesis (4).
Probe labeling using replacement synthesis (1,2).

Product Overview

Product Image

T4 DNA Polymerase

by New England Biolabs Inc.

3.9/5.0 (5 reviews)
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Receive your quote directly from New England Biolabs Inc. for T4 DNA Polymerase