T4 DNA Polymerase

T4 DNA Polymerase
by New England Biolabs Inc.
 3.9/5.0 (5 reviews)

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function. Source: Purified from a strain of E. coli that carries a T4 DNA Pol...read more

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Reviews

AVERAGE RATING: 3.9

5 scientists have reviewed this product

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  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY

Kim Wu


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Member since 2013

Organization:
AstraZeneca
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 07 Oct 2013

Application Area: DNA purification
T4 DNA Polymerase

"It is easy to use, the protocol is simple and quick. DNA purification can be used in a wide area of research, could led to finding potential protein that is coded from DNA."

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Masahiro Onozawa


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Member since 2013

Organization:
Ccr, Nci, Nih
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RATING: 3.7

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 06 Aug 2013

Application Area: leukemia genetics
T4 DNA Polymerase

"I use this for sub cloning PCR product. It's always reliable and powerful enough to get enough yield. Efficiency seems more time-dependent, sometimes I get low yield when I incubate just for a short time < 1hr."

Joseph Carver


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Member since 2013

Organization:
Nih
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: DNA cloning
T4 DNA Polymerase

"Excellent consistency and long term quality. This product works well with NEB's other products - I would recommend their entire line of enzymes to my colleagues."

Wayne A. Warner


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Member since 2013

Organization:
California State Univ.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: ligation
T4 DNA Polymerase

"Excellent product and great website. Company provides great service. Works well. Good value for money. Highly recommended. I have been using it for a while and it works every time."

Bin Wu


Status:


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Member since 2010

Organization:
Albert Einstein College Of Medicine
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RATING: 2.0

  • 3 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 1 out of 5VALUE FOR MONEY




Review date: 05 Jan 2011

T4 DNA Polymerase

"Not good."

Description

 

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid.

Applications:
3´ overhang removal to form blunt ends (1,2).
5´overhang fill-in to form blunt ends (1,2).
Single strand deletion subcloning (3).
Second strand synthesis in site-directed mutagenesis (4).
Probe labeling using replacement synthesis (1,2).

Product Overview

T4 DNA Polymerase by New England Biolabs Inc.
T4 DNA Polymerase




  • 4out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY





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