T4 DNA Polymerase by New England Biolabs Inc.

3.9
/
5.0
  |  5 reviews
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function. Source: Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid. Applications: 3´ overhang removal to form blunt ends (1,2). 5´overhang fil... Read more


T4 DNA Polymerase by New England Biolabs Inc. product image
T4 DNA Polymerase

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Average Rating: 3.9
5 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 4.0

  • Application Area: DNA purification

"It is easy to use, the protocol is simple and quick. DNA purification can be used in a wide area of research, could led to finding potential protein that is coded from DNA."

Review date: 07 Oct 2013 | T4 DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Ccr, Nci, Nih



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.7

  • Application Area:leukemia genetics

"I use this for sub cloning PCR product. It's always reliable and powerful enough to get enough yield. Efficiency seems more time-dependent, sometimes I get low yield when I incubate just for a short time < 1hr."

Review date: 06 Aug 2013 | T4 DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Nih



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:DNA cloning

"Excellent consistency and long term quality. This product works well with NEB's other products - I would recommend their entire line of enzymes to my colleagues."

Review date: 03 Jun 2013 | T4 DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: California State Univ.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:ligation

"Excellent product and great website. Company provides great service. Works well. Good value for money. Highly recommended. I have been using it for a while and it works every time."

Review date: 03 Jun 2013 | T4 DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Albert Einstein College Of Medicine



  • Ease of use
    3 out of 5
    After sales service
    2 out of 5
    Value for money
    1 out of 5
Rating: 2.0
"Not good."

Review date: 05 Jan 2011 | T4 DNA Polymerase
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid.

Applications:
3´ overhang removal to form blunt ends (1,2).
5´overhang fill-in to form blunt ends (1,2).
Single strand deletion subcloning (3).
Second strand synthesis in site-directed mutagenesis (4).
Probe labeling using replacement synthesis (1,2).