SYNTHETIC OLIGONUCLEOTIDE 5′-END LABELING KIT For use with [γ-32P ATP], 30 assays by MP Biomedicals
For 10 or 30 ReactionsThe MP DNA 5´-End Labeling Kit is a complete system for simple and rapid labeling of both DNA fragments and synthetic nucleotides. T4 polynucleotide kinase catalyzes the transfer of the γ-phosphate of [γ-32P]-ATP to the 5´-end of DNA or oligonucleotide. DNA fragments may be kinased with a radioactive phosphate by exchange reaction or may be dephosphorylated with alkaline phosphatase and end-labeled by direct reaction. The kit features optimized buffers for labeling 5´-protr...
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