Status:
Expert ReviewerMember since: 2017
Organization: Punjab Agricultural University
Run many reactions in a day so a great product. I love it.
Application Area: Insect gene expression, RNAi studies
"This is a wonderful product The advantage of this product over others is that it takes 40-50 minutes for a reaction with this mix."
Status:
ReviewerMember since: 2016
Organization: IITM
Robust enzyme and reproducible and fast reaction settings.
Application Area:Gene expression analysis by qRT-PCR
"Very good product with high efficiency and accuracy. Partly due to the RNase. No worries about residual RNA present and due to the fast amplification ability of the polymerase, cycle times are less and the reaction finishes faster."
The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly; by simply adding primers, template and sterile distilled water, intercalator-based qPCR can be performed.
TB Green Premix Ex Taq (Tli RNase H Plus) contains TaKaRa Ex Taq HS, a hot start PCR enzyme that includes an anti-Taq antibody. The TaKaRa Ex Taq HS enzyme, in combination with its qPCR optimized buffer, allows for high amplification efficiency and detection sensitivity in qPCR. The 2X TB Green qPCR premix also contains Tli RNase H, a heat-resistant RNase H that minimizes qPCR inhibition as a consequence of residual mRNA in the input template cDNA. This product enables accurate detection of targets even during high-speed reactions, making it possible to conduct fast RT-PCR (qPCR) analyses and achieve excellent reproducibility and reliability.
Features:
Applications: