Fatty matrix is preferentially retained by the cartridge while PAHs are washed through using acetonitrile. The resulting extract is suitable for either GC-MS or HPLC-FLD analysis. The top layer of the SPE cartridge consists of Florisil®, a polar adsorption media that interacts with polar functional groups such as acids and alcohols. The bottom layer is comprised of a mixture of Discovery® DSC-18 and Z-Sep sorbents. C18 binds fatty matrix through hydrophobic interaction. Z-Sep, a zirconia coated silica, acts as a Lewis acid, interacting with the oxygen within OH and COOH groups found in fatty acids and mono and diacylglycerols.
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