DH5α™ is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
DH5α™ Cells offer the following benefits:
A wide range of efficiencies from >1 x 10^6 to >1 x 10^9 transformants/µg
Greatly increased plasmid yield and quality due to endA1 mutation
Blue/white screening of recombinant clones due to lacZΔM15