StrataClone Blunt PCR Cloning Kit

StrataClone Blunt PCR Cloning Kit
by Agilent Technologies
 5.0/5.0 (1 review)

The StrataClone PCR Cloning Kits are a topoisomerase-based cloning method. Kits are available for both blunt-end and UA cloning. We have combined the highest accuracy in PCR amplification with easy, reliable, and affordably priced topoisomerase-based cloning in our StrataClone Ultra PCR Cloning System, which includes the PfuUltra II Fusion HS DNA Polymerase.  Features DNA topoisomerase I and Cr...read more

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Reviews

AVERAGE RATING: 5.0

1 scientist has reviewed this product

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  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY

Paul Gao


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Member since 2011

Organization:
Memorial Sloan-Kettering Cancer Center
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 15 Dec 2014

Application Area: To Clone High Fidelity PCR products into a vector
StrataClone Blunt PCR Cloning Kit

"I love this kit! Especially when I am doing CRISPR, that means I need to subclone the high fidelity amplified genomic locus sequence, to check the intended mutations, this is the only kit I use! Way better than other company's products, works every time! "

Description

 

The StrataClone PCR Cloning Kits are a topoisomerase-based cloning method. Kits are available for both blunt-end and UA cloning. We have combined the highest accuracy in PCR amplification with easy, reliable, and affordably priced topoisomerase-based cloning in our StrataClone Ultra PCR Cloning System, which includes the PfuUltra II Fusion HS DNA Polymerase.

  •  Features DNA topoisomerase I and Cre recombinase technologies
  •  With Kanamycin and Ampicillin Resistance Markers
  •  Clone both long and short amplicons with the same kit
  •  Simple, three step protocol with 5-minute room temperature ligation
  •  StrataClone Ultra PCR Cloning Kits contain High efficiency yields >95% insert positive clones
  •  StrataClone Ultra PCR Cloning Kits contain     PfuUltra    II Fusion HS DNA Polymerase

Product Overview

StrataClone Blunt PCR Cloning Kit by Agilent Technologies
StrataClone Blunt PCR Cloning Kit




  • 5out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY





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