StepOnePlus™ Real-Time PCR System

Manufacturer Applied Biosystems
4.2
/
5.0
  |  25 reviews


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Average Rating: 4.2
25 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Wonderful instrument to use.
Rating: 5.0

  • Application Area: Genotyping

"User friendly, valuable instrument for our research efforts."

Review date: 12 Jul 2016 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Wayne%20State%20University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use and versatile real-time PCR machine
Rating: 5.0

  • Application Area:Real time PCR and RT PCR

"SetpOnePlus is really easy to use real-time PCR machine. This machine can be operated in standalone mode as well as with connected PC. Further, it can be linked to email server to receive remote notifications related to assay run. Heating element on this machine is separated in two columns (i.e. total 6 blocks). Each heat block can be operated at different heating temperature profile. It works really nice with customized assay with different primer annealing temperatures. Similarly, it can also accommodate multiplexing with 5 different detection sources. Besides the real-time PCR, this machine is capable of running various different types of pre-designed as well as customized nucleic acid assays. "

Review date: 31 May 2016 | StepOnePlus™ Real-Time PCR System
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: Newcastle University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Highly recomended
Rating: 3.7

  • Application Area:Quantitative analysis of DNA

"An extremely compact, feature-rich, user-friendly qPCR machine with an impressive software package. "

Review date: 31 Mar 2016 | StepOnePlus™ Real-Time PCR System
  • Status:

    Advanced Reviewer

  • Member since: 2014

  • Organization: Imperial College London



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"Compact unit, very easy to use, and does the job. Service is okay, if breaks down, replacements are sent in efficiently. "

Review date: 15 Dec 2014 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: MHH- Hannover Medical School



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Gene expression system

"It is very easy to use with gene expression systems. Operation is quite user friendly. The software is quite basic however it makes it easy to analyze the data. "

Review date: 06 Nov 2013 | StepOnePlus™ Real-Time PCR System


  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Ucsd



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:UCSD

"We have been using this qPCR machine since I've been in lab, and it has been working very well for us. It is quite user friendly and offers a wide range of data analysis tools."

Review date: 03 Jun 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Univ. Of Illinois At Chicago



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:prostate cancer

"Machine was a great addition to our lab. Very easy to use. The software is user friendly and intuitive. The small footprint is great as it does not occupy a lot of space on the bench. Customer service from Applied Biosystems has been great."

Review date: 03 Jun 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: MEDICAL UNIVERSITY OF VIENNA



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:RNA extraction

"Excellent services, not many problems experienced. Good after sales support, and online data representation."

Review date: 03 Jun 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: DREXEL UNIV.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:qRT-PCR

"This is a very easy system to work on qRT-PCR. Very good and compatible primers. Easy online ordering."

Review date: 03 Jun 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: University of Queensland



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Real time PCR

"StepOnePlus is a very easy-to use high-throughput instrument. It’s very fast, efficient, user friendly and precise."

Review date: 15 Jan 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: FMI



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Biochemistry

"The software and hardware for this system is good and user-friendly. Also, the calibration settings are well designed. Furthermore, it is possible to run many analyses."

Review date: 03 Jan 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: FMI



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Biology

"This kit is very convenient to use and setting up reactions is easy. Therefore, I would recommend this to my colleagues."

Review date: 03 Jan 2013 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: Pontificia Universidad Catolic



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Gene Expression

"It is easy to use and you can use it to assess a single gene or biological group of interest. Using the VeriFlex option it is very useful for performing temperature gradient assays. Also, it's possible to do high resolution melt analysis with a different set."

Review date: 26 Nov 2012 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: THOMAS JEFFERSON UNIV.



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.0

  • Application Area:Real time PCR

"The software is very user friendly."

Review date: 06 Jun 2012 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: YALE CANCER CTR.



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Rating: 4.3

  • Application Area:PCR Molecular biology

"Easy to use, sophisticated and good value for money. Produces a quick result with simple sample preparation. Quick response from service."

Review date: 06 Jun 2012 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: University of Bergen



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    2 out of 5
Rating: 2.7

  • Application Area:qPCR

"Easy for analysis and convenient for 96 well plates. However the reagents are expensive."

Review date: 06 Jun 2012 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: Isis Pharmaceuticals



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Intuitive and easy to use software. Ability to program custom settings. Can be used for taq-man, cyber- green etc. Able to use a variety of reagents- not Applied Biosystems reagent specific."

Review date: 29 Feb 2012 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: Monterey Bay Aquarium Research Institute



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Environmental sample analysis

"Good software, easy to use. Very portable, can be moved without damaging detector- usable at sea. I have compared different models, this one is much easier to program and use. The block run in different temperature zones make optimization easier."

Review date: 23 Feb 2012 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: VANDERBILT UNIVERSITY



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"Very easy to use."

Review date: 25 Jul 2011 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: WAYNE STATE UNIVERSITY



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Easy to use, perfect results, intuitive software."

Review date: 15 Jul 2011 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: MASSACHUSETTS GENERAL HOSPITAL



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Easy to use, matched reagent, good tech support."

Review date: 08 Jul 2011 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: UNIV. OF WISCONSIN-MADISON



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"User friendly machine. Software very easy to use and understand. The machine works very well."

Review date: 08 Jul 2011 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Chang Gung University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"Good."

Review date: 21 Jan 2011 | StepOnePlus™ Real-Time PCR System
  • Status:

    Advanced Reviewer

  • Member since: 2007

  • Organization: Institute of Cell Biology and Pathology



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

Review date: 19 Jan 2011 | StepOnePlus™ Real-Time PCR System
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Centro Investigacien



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"It's the best."

Review date: 24 Nov 2010 | StepOnePlus™ Real-Time PCR System
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users.

Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

Instrument software for the Applied Biosystems StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis.
The StepOnePlus™ system brings advanced real-time PCR technology to a new level of accessibility. Beginning at the StepOne software homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection.

The software is even pre-populated with the right run protocol for each application when using reagents from Applied Biosystems. Just enter your plate set up and go. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. The StepOnePlus System is a long life LED based optical system that records fluorescence from FAM/SYBR Green, VIC/JOE, NED/TAMRA and ROX dyes for gene expression analysis, pathogen detection, SNP genotyping and presence/absence assays. This 4-color/96 well system delivers precise, quantitative real-time PCR results that you can have total confidence in.

StepOnePlus™ Real-Time PCR System Features:

  • Advanced software and instrumentation for performing a wide array of genomic assays.
  • Sensitive 4-color optical LED recording system.
  • Intuitive and robust software perfect for first-time and advanced users.
  • Simple and flexible instrument set-up and usage.
  • Rapid and simple assay set-up.
  • Versatile instrument configuration options.
  • Energy efficiency and space saving.
  • Real-time data monitoring, dissemination and storage.
  • Advanced High Resolution Melting Capability

Product Overview

Product Image

StepOnePlus™ Real-Time PCR System

Manufacturer Applied Biosystems

4.2 / 5.0 | 25 reviews