- Ease of use
- After sales service
- Value for money

Description
Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
Instrument software for the Applied Biosystems StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis.
The StepOnePlus™ system brings advanced real-time PCR technology to a new level of accessibility. Beginning at the StepOne software homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection.
The software is even pre-populated with the right run protocol for each application when using reagents from Applied Biosystems. Just enter your plate set up and go. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. The StepOnePlus System is a long life LED based optical system that records fluorescence from FAM/SYBR Green, VIC/JOE, NED/TAMRA and ROX dyes for gene expression analysis, pathogen detection, SNP genotyping and presence/absence assays. This 4-color/96 well system delivers precise, quantitative real-time PCR results that you can have total confidence in.
Reviews
Iman Azimi![]() Status: See all reviews Member since 2013 Organization: University of Queensland Read more » | RATING: 4.7 ![]()
"StepOnePlus is a very easy-to use high-throughput instrument. It’s very fast, efficient, user friendly and precise." |
Anand Manoharan![]() Status: See all reviews Member since 2013 Organization: FMI Read more » | RATING: 4.0 ![]()
"The software and hardware for this system is good and user-friendly. Also, the calibration settings are well designed. Furthermore, it is possible to run many analyses." |
Jayanthi Ganesan![]() Status: See all reviews Member since 2013 Organization: FMI Read more » | RATING: 4.0 ![]()
"This kit is very convenient to use and setting up reactions is easy. Therefore, I would recommend this to my colleagues." |
Pablo Arroyo![]() Status: See all reviews Member since 2012 Job Title: Research Scientist / Senior / PI Discipline: 26 Work Field: Academia / University Organization: Pontificia Universidad Catolic Read more » | RATING: 4.7 ![]()
"It is easy to use and you can use it to assess a single gene or biological group of interest. Using the VeriFlex option it is very useful for performing temperature gradient assays. Also, it's possible to do high resolution melt analysis with a different set." |
SHAO-GUI WAN![]() Status: See all reviews Member since 2010 Organization: THOMAS JEFFERSON UNIV. Read more » | RATING: 3.0 ![]()
"The software is very user friendly." |
SRINIVAS KODURU![]() Status: See all reviews Member since 2010 Organization: YALE CANCER CTR. Read more » | RATING: 4.3 ![]()
"Easy to use, sophisticated and good value for money. Produces a quick result with simple sample preparation. Quick response from service." |
Sunita Sharma![]() Status: See all reviews Member since 2012 Organization: University of Bergen Read more » | RATING: 2.6 ![]()
"Easy for analysis and convenient for 96 well plates. However the reagents are expensive." |
Melanie Katz![]() Status: See all reviews Member since 2012 Organization: Isis Pharmaceuticals Read more » | RATING: 4.3 ![]()
"Intuitive and easy to use software. Ability to program custom settings. Can be used for taq-man, cyber- green etc. Able to use a variety of reagents- not Applied Biosystems reagent specific." |
Chris Preston![]() Status: See all reviews Member since 2009 Organization: Monterey Bay Aquarium Research Institute Read more » | RATING: 4.3 ![]()
"Good software, easy to use. Very portable, can be moved without damaging detector- usable at sea. I have compared different models, this one is much easier to program and use. The block run in different temperature zones make optimization easier." |
JAMIE AUSBORN![]() Status: See all reviews Member since 2011 Organization: VANDERBILT UNIVERSITY Read more » | RATING: 4.0 ![]()
"Very easy to use." |
Vitaly Balan![]() Status: See all reviews Member since 2009 Organization: WAYNE STATE UNIVERSITY Read more » | RATING: 5.0 ![]()
"Easy to use, perfect results, intuitive software." |
ZHENFENG DUAN![]() Status: See all reviews Member since 2011 Organization: MASSACHUSETTS GENERAL HOSPITAL Read more » | RATING: 4.3 ![]()
"Easy to use, matched reagent, good tech support." |
NITYANAND MADDODI![]() Status: See all reviews Member since 2011 Organization: UNIV. OF WISCONSIN-MADISON Read more » | RATING: 4.3 ![]()
"User friendly machine. Software very easy to use and understand. The machine works very well." |
Chuen-Mao Yang![]() Status: See all reviews Member since 2007 Organization: Chang Gung University Read more » | RATING: 4.0 ![]()
"Good." |
Loredan Stefan Niculescu![]() Status: See all reviews Member since 2007 Organization: Institute of Cell Biology and Pathology Read more » | RATING: 4.6 ![]()
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Carla Angulo Rojo![]() Status: See all reviews Member since 2010 Organization: Centro Investigacien Read more » | RATING: 5.0 ![]()
"It's the best." |
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