The StemElite™ ID System provides the reagents required for successful and simple identification and authentication of human cell lines and detection of intra-species cell line cross-contamination. The StemElite™ ID System uses short tandem repeat (STR) analysis of specific loci in the human genome through co-amplification and three-color detection of ten loci (eight STR loci from PowerPlex® 1.2, plus D21S11 and Amelogenin for gender identification). Additionally, the StemElite™ ID System incorporates a sensitive marker that specifically detects the presence of DNA from mouse (Mus musculus).
All eleven loci are amplified simultaneously in a single tube. The StemElite™ ID System includes a hot-start Taq DNA polymerase system in an enzyme mix for convenient room-temperature reaction assembly. Alleles, along with the allelic ladder and an internal lane standard that assists in defining sizes for each of the loci, are resolved by capillary electrophoresis in a single injection, and CE data are analyzed using allele-calling software to generate a genetic profile of the cell line. These loci collectively provide a genetic profile with a random match probability of 1 in 2.92 × 109.