SsoAdvanced™ SYBR® Green supermix is the newest high–performance real–time PCR supermix based on Bio–Rad's patented* Sso7d fusion protein technology. It is formulated for a wide range of qPCR applications. The dsDNA binding protein, Sso7d, stabilizes the polymerase–template complex, providing superior inhibitor tolerance, increased processivity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility. Key Features and Benefits of Green Supermix: Achieve superior gene ... Read more
SsoAdvanced SYBR Green Supermix (172–5260)
Average Rating: 4.8
6 Scientists have reviewed this product
Status:
Reviewer
Member since: 2017
Organization: Case Western Reserve University
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- Ease of use
- After sales service
- Value for money
Awesome SyBr Mix for RT-PCR.
Rating: 5.0
Application Area: qRT-PCR, gene expression analysis
"Performed RT-PCR with this Mix. It is easy to use. I actually use lesser volume than our conventional SyBr Mix, aka, I can use it longer and for more samples. CT values and amplification was accurate!"
Review date: 30 Nov 2018 | SsoAdvanced SYBR Green Supermix (172–5260)
Status:
Reviewer Member since: 2016
Organization: Vanderbilt University Medical Center
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- Ease of use
- After sales service
- Value for money
I love it
Rating: 5.0
Review date: 22 Dec 2016 | SsoAdvanced SYBR Green Supermix (172–5260)
Application Area:This SYBR Mix is a lot cheaper than others and e itworks perfect. i love it.
"This SYBR Mix is a lot cheaper than others and works easier and faster . I love it"
Review date: 22 Dec 2016 | SsoAdvanced SYBR Green Supermix (172–5260)
Status:
Reviewer Member since: 2015
Organization: Johns Hopkins University
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- Ease of use
- After sales service
- Value for money
Rating: 5.0
Review date: 17 Apr 2015 | SsoAdvanced SYBR Green Supermix (172–5260)
Application Area:Low volume gene expression qPCR
"I've found that SsoAdvanced has lived up to the promise. I get excellent melt curves, CVs, and reaction efficiencies with this mix. I've set up (with liquid handling) 2 microliter reactions (cycled on the CFX384), left replicate plates sitting at room temperature and had <1% intrarun Cq CVs. Excellent product!"
Review date: 17 Apr 2015 | SsoAdvanced SYBR Green Supermix (172–5260)
Status:
Advanced Reviewer Member since: 2012
Organization: Fidia Pharmaceuticals
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- Ease of use
- After sales service
- Value for money
Rating: 4.3
Review date: 23 Jan 2015 | SsoAdvanced SYBR Green Supermix (172–5260)
"Another great product from BioRad! As a qPCR user I look for fast, accurate, repeatable results and this master mix really meets all of these requirements. The stable polymerase:template complex created provides greater speed due to reduced reaction times (1-5 sec annealing) and the fluorescent molecule EvaGreen generates great fluorescent signal and provides increased sensitivity that you will not feel the lack of SYBR green. "
Review date: 23 Jan 2015 | SsoAdvanced SYBR Green Supermix (172–5260)
Status:
Expert Reviewer Member since: 2010
Organization: Penn State University
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- Ease of use
- After sales service
- Value for money
Rating: 5.0
Review date: 17 Dec 2014 | SsoAdvanced SYBR Green Supermix (172–5260)
"Being new to real time procedures, this product helped with keeping things simple and economical. Like every other Bio-Rad product, quality is the best! If you have used iSYBR before, this one is even better."
Review date: 17 Dec 2014 | SsoAdvanced SYBR Green Supermix (172–5260)
Status:
Advanced Reviewer Member since: 2010
Organization: Lovelace Respiratory Research Institute
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- Ease of use
- After sales service
- Value for money
Rating: 4.7
Review date: 08 Jan 2014 | SsoAdvanced SYBR Green Supermix (172–5260)
Application Area:PCR
"This SYBR Mix is a lot cheaper than others out there and works just fine. I had a free test sample a while back and it works just as well as other brands, if not better."
Review date: 08 Jan 2014 | SsoAdvanced SYBR Green Supermix (172–5260)
SsoAdvanced™ SYBR® Green supermix is the newest high–performance real–time PCR supermix based on Bio–Rad's patented* Sso7d fusion protein technology. It is formulated for a wide range of qPCR applications. The dsDNA binding protein, Sso7d, stabilizes the polymerase–template complex, providing superior inhibitor tolerance, increased processivity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility. Key Features and Benefits of Green Supermix: Achieve superior gene expression results under various cycling conditions — robust formulation delivers consistent performance in standard or fast cycling Increase sensitivity and efficiency of detection from compromised samples — Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors Decrease run times and time to results without compromising qPCR data quality — Sso7d fusion polymerase and optimized buffer provide rapid polymerization kinetics and instant polymerase activation Obtain better results with predeveloped qPCR assays — advanced formulation tolerates a broad range of reaction conditions, primer concentrations, and temperature ranges * U.S. patents 6,627,424; 7,541,170; and 7,560,260.
