SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml by Bio-Rad

Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.

• Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
• Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
• For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays , self-designed assays, and TaqMan Assays

Product Comparison

* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

• iScript™ Reverse Transcription Supermix for RT-qPCR
• iScript™ Advanced cDNA Synthesis Kit for RT-qPCR

qPCR Supermixes

• SsoAdvanced™ Universal SYBR^® Green Supermix
• SsoAdvanced™ Universal Probes Supermix

PreAmp and qPCR Assays

• PrimePCR™ PreAmp Assays
• PrimePCR™ Assays

More Information

Find out more about the features of the SsoAdvanced™ PreAmp Supermix .