The CHEMICON ssDNA Apoptosis ELISA Kit is a convenient, sensitive method for early detection of apoptosis. The assay can be performed in two formats:
1. Cells are grown, treated with apoptosis inducing agents, stained and analyzed in the same microtiter plate. This assay is suitable for high throughput screening when induction of apoptosis is used as the endpoint of drug activity.
2. Cell suspensions obtained from cultures or tissues are transferred into a microtiter plate for staining and analysis. For this assay, cells could be fixed with methanol and stored before transfer into plates. Alternatively, fixation could be performed in microtiter plates after transfer of non-fixed cells.
Total assay time is 3-4 hours, which includes fixation and staining. Only one washing step is required.
The ssDNA Apoptosis ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.