The vial contains a mixture of 1.7 – 2.2 µm Yellow Fluorescent Particles that are dyed to have an excitation maximum of approximately 470 nm with a subsequent emission maximum of approximately 480 nm. This particle mixture is useful with flow cytometers equipped with a 488-nm argon laser. This particle mixture has compatible excitation and emission spectra with the fluorescein isothiocyanate (FITC) fluorophore.
Preparation and Storage
The particle mixture (~ 1.0% w/v) should be stored undiluted at 4°C and protected from direct exposure to light. Do not freeze.
This particle mixture is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis. Since applications vary, each investigator must determine dilutions appropriate for individual use.