The vial contains a mixture of 1.7 – 2.2 µm UV Fluorescent Particles that are dyed to have an excitation maximum of approximately 360 nm with a subsequent emission maximum of approxim...read more
The vial contains a mixture of 1.7 – 2.2 µm UV Fluorescent Particles that are dyed to have an excitation maximum of approximately 360 nm with a subsequent emission maximum of approximately 415 nm. This particle mixture is useful with flow cytometers equipped with a multi-line UV laser. This particle mixture has compatible excitation and emission spectra with the Hoechst 33342 and 33258 fluorophores.
Preparation and Storage
The particle mixture (~ 1.0% w/v) should be stored undiluted at 4°C and protected from direct exposure to light. Do not freeze.
This particle mixture is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis. Since applications vary, each investigator must determine dilutions appropriate for individual use.
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on March 30th 2014).
Join the Global SelectScience Community Today! It’s FREE!
Helping you make informed decisions about the latest lab products and technologies:
Be the first to learn about new technologies
10,000+ trusted product reviews from your peers
Compare 500,000 products and manufacturers
Get exclusive buying tips from experts
Find solutions fast: 10,000+ Application Notes, Webinars and Videos
Connect with 250,000 scientists... All in one place!