Sphero™ UV (Low Intensity) Fluorescent Particles, 1.7-2.2 mm

Manufacturer BD Biosciences

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The vial contains a mixture of 1.7 – 2.2 µm UV Fluorescent Particles that are dyed to have an excitation maximum of approximately 360 nm with a subsequent emission maximum of approximately 415 nm. This particle mixture is useful with flow cytometers equipped with a multi-line UV laser. This particle mixture has compatible excitation and emission spectra with the Hoechst 33342 and 33258 fluorophores.

Preparation and Storage

The particle mixture (~ 1.0% w/v) should be stored undiluted at 4°C and protected from direct exposure to light. Do not freeze.


This particle mixture is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis. Since applications vary, each investigator must determine dilutions appropriate for individual use.