The vial contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity. The intensity of this particle corresponds to the brightest intensity peak in the Rainbow Calibration Particles, 3.0 - 3.4 µm (Cat. no. 556286). Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from 365 – 650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.
Preparation and Storage
The particle mixture (~ 10 x 106 particles/ml) should be stored undiluted at 4°C and protected from direct exposure to light. Do not freeze.
This particle mixture is useful for routine calibration of flow cytometers.
Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis. Since applications vary, each investigator must determine dilutions appropriate for individual use.