SNAP i.d. 2.0 System

SNAP i.d. 2.0 System
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by EMD Millipore, a division of Merck KGaA, Darmstadt, Germany
 4.3/5.0 (14 reviews)

The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time! Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d. system applies a vacuum to actively drive reagents through the membrane. This novel method allows you to optimize your blotting conditions in record time for maximum results. T...read more

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Reviews

AVERAGE RATING: 4.3

14 scientists have reviewed this product

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  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY

Robert Best


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Member since 2013

Organization:
Elkay Corp
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 19 Nov 2014

SNAP i.d. 2.0 System

"This product is exceptionally easy to use, especially for those who have little experience quantifying. This product is worth trying if you haven't already done so. "

Read 14 reviews

Arunkumar Renganathan Chandrik


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Member since 2013

Organization:
Rajiv Gandhi Centre For Biotechnology
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RATING: 3.3

  • 5 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 02 Dec 2013

Application Area: Western Blot Of Proteins Like CaMKII
SNAP i.d. 2.0 System

"The SNAP-id from Millipore is very good at reducing the time required for your blot development but it ruthlessly consumes your antibody. Also there is a need for extra consumables which costs too much. The dry blot system from invitrogen also suffers the same problem even though it has the added advantage of reducing the gel transferring time also."

Katie Anderson


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Member since 2012

Organization:
University of Kentucky
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 02 Dec 2013

Application Area: Western Blotting
SNAP i.d. 2.0 System

"I use this because of the time savings in incubating my membranes. It uses 3x the antibody that passive incubation does, but you can recover and re-use the antibody so it's best used when you have many membranes (lots of samples for testing). You do need to buy folders for each membrane but again the time savings is worth it. It also is great for the rotating grad students and undergrad volunteers who have less time to complete westerns. "

Stephanie Shishido


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Member since 2013

Organization:
Kansas State Univ.
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

SNAP i.d. 2.0 System

"This system is fast and easy to use. It does require a larger concentration of antibodies, but the signal has always been strong. The casettes are not reusable, but are not too expensive."

Cristina Rohena


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Member since 2010

Organization:
Uthscsa
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RATING: 3.0

  • 4 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: Protein expression
SNAP i.d. 2.0 System

"This initially seemed like a good idea, but there are too many limitations association with its use. The membranes are too small for the unit and I don't get even signal."

Suzanne Lababidi


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Member since 2012

Organization:
Neomed
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 16 Dec 2012

Application Area: Western Blots
SNAP i.d. 2.0 System

"This is a fantastic system that allows you to spend hours, not days, performing a Western Blot. The amount of time saved easily makes up for the cost. The only downside is the cassettes are not entirely reusable and are somewhat pricy."

Ciric To


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Member since 2011

Organization:
UNIV. OF WESTERN ONTARIO
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RATING: 2.6

  • 2 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 31 May 2012

Application Area: Western blotting
SNAP i.d. 2.0 System

"The idea of the system is great in that it allows you to detect protein levels in ~2 hours, hence saving a lot of time. However, in my experience, this system only works on certain antibodies and the quality of the data obtained from the system is poor (hazy, decreased signal compared to blots processed in the traditional way). The system utilizes a vacuum method to accelerate the time it takes to get results, but we have found that the vacuum clogs a lot of the time. After sales service were not helpful- they couldn't really help us troubleshoot and didn't provide any suggestions."

Melissa Wason


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Member since 2011

Organization:
UCF
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 26 Jul 2011

SNAP i.d. 2.0 System

"Not as clear as traditional method but saves a lot of time and is great for screening."

Chris Lee


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Member since 2011

Organization:
Orlando Health
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 26 Jul 2011

SNAP i.d. 2.0 System

"Optimization takes time, cassettes can be reused, perfect for screening."

Angela Hess


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Member since 2010

Organization:
Bloomsburg Univ
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 26 Jan 2011

SNAP i.d. 2.0 System

"This is a fantastic product for Western Blot probing. It has saved my lab significant time and money."

Stephanie C.


Status:


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Member since 2007

Organization:
Univ California Davis
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Jan 2011

SNAP i.d. 2.0 System

"Very easy to use, quick too!"

Stephanie Crockett


Status:


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Member since 2010

Organization:
UC Davis
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 19 Jan 2010

SNAP i.d. 2.0 System

"I love this product!"

Donna Hill


Status:


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Member since 2010

Organization:
University Of Alabama At Birmingham
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 19 Jan 2010

SNAP i.d. 2.0 System

Beverly Wuertz


Status:


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Member since 2009

Organization:
University of Minnesota
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 18 May 2009

SNAP i.d. 2.0 System

"Very easy to use and saves on reagents. It also saves a huge amount of time over traditional antibody incubations."

Description

 

The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time!

Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d. system applies a vacuum to actively drive reagents through the membrane. This novel method allows you to optimize your blotting conditions in record time for maximum results. The SNAP i.d. system minimizes overblocking by using lower concentrations of blocking agents. In addition, more effective wash steps remove unwanted contaminants from the membrane.

With the SNAP i.d. system, you’ll achieve incredibly low background, high signal-to-noise ratios, reproducibility from blot to blot, and sensitivities that are the same or better than traditional immunodetection techniques. Compatible with your favorite membrane and detection methods, the SNAP i.d. system produces higher quality blots than traditional western blot methods -- in less than 30 minutes.


SNAP i.d. System Features:

  • Dynamic – vacuum actively drives reagents through blotting membrane.
  • Quality – equal or better signal-to-noise ratios than standard western blotting.
  • Fast – reduces immunodetection time from 4 hours to 30 minutes.
  • Simple – incorporates blocking, washing, and antibody incubation steps.
  • Compatible – works with standard gel sizes and protocols.
  • Efficient – optimizes your protocol with a new antibody in 30 minutes.

Product Overview

SNAP i.d. 2.0 System by EMD Millipore, a division of Merck KGaA, Darmstadt, Germany
SNAP i.d. 2.0 System




  • 5out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY





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