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Status:
Reviewer
Member since: 2021
Organization: UCLA, Jules Stein Eye Institute
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- Ease of use
- After sales service
- Value for money
Great results.
Application Area: RNA-seq library prep
"Yield good results in my RNA-seq data which uses tiny amount of tissue from mice."
Review date: 21 Jun 2021 | SMART-Seq® v4 Ultra Low Input RNA Kit
SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing—Single-Cell Transcriptome Analysis with Ultimate Sensitivity
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is the fourth-generation of SMARTer Ultra Low kits, and our most sensitive mRNA-seq kit for single cells and ultra-low inputs. This kit takes SMART (Switching Mechanism at 5’ End of RNA Template) technology to the next level by incorporating and improving upon the SMART-Seq2 method and locked nucleic acid (LNA) technology. With these additions—referred to as ""SMARTer-Seq® chemistry""—this kit is able to outperform both the SMART-seq2 method and previous generations of SMARTer kits by identifying the greatest number of transcripts. The Clontech tradition of continuous innovation extends to this kit by providing direct cDNA synthesis from as few as 1–1,000 intact cells (or as little as 10 pg–10 ng of total RNA), high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts. The full-length cDNA libraries from this kit are compatible with both Illumina® and Ion Torrent sequencing platforms.
The core SMART technology in this kit is an immensely powerful tool for studying the full transcriptome, including splice junctions and alternative splicing events in cells, and provides full-length gene coverage. Oligo(dT) priming is used to specifically amplify mRNA from either high-quality total RNA (RIN >8) or intact cells. LNA technology improves the efficiency of template switching, which increases the number of genes identified to higher levels than any other SMARTer Ultra Low kit for mRNA-seq.
Information about prior SMARTer Ultra Low RNA kits can be found here.
Features:
- SMART-Seq2 and LNA technologies—Template switching efficiency is improved by building on LNA technology
- Unparalleled sensitivity—Start with as little as 1 cell or 10 pg of total RNA (input range: 1–1,000 cells or 10 pg–10 ng of total RNA)
- High-quality RNA-seq data—Identify the highest number of genes of any SMARTer Ultra Low kit; maintain full-length gene information, low percentage of rRNA reads, and great representation of GC-rich transcripts
- Suitable for multiple sequencing platforms—cDNA libraries are compatible with either Illumina or Ion Torrent NGS platforms
- Sample prep made easy—The single-tube protocol works directly on whole cells and helps to preserve sample integrity
- Interested in more data and FAQs about this product? Visit the NGS Resource Portal
Applications:
- cDNA synthesis from small quantities of cells or total RNA for transcriptome sequencing
- cDNA output from this kit can be used with either our Low Input Library Prep Kit or Nextera® XT library preparation kits for Illumina sequencing, or with the Ion Xpress Plus Fragment Library Kit for Ion Torrent sequencing
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