Status:
ReviewerMember since: 2015
Organization: Eurofins Lancaster Laboratories
Provide quick, robust and convenient application
Application Area: Peptide map analysis using tryptic digests
"The Smart Digest using immobilized trypsin provides a quick and convenient digestion tool for monoclonal antibodies (in 15-30 min). If full coverage is not a goal, no need for additional reduction/alkylation. Resulting peptide mixture can be used for peptide map analysis or glycosylation analysis focusing on glycopeptides. With post-digestion reduction/alkylation full coverage can be achieved."
Status:
Advanced ReviewerMember since: 2014
Organization: The University of Warwick
Application Area:quick digestion of proteins
"Ideal when looking at PTM's in the target proteins. Longer peptide generation protocols, that can be cheaper per sample, will inevitably introduce unwanted PTM's, especially oxidation of methionine that can interfere with downstream analysis. Not ideal for phosphopeptide analysis as the buffers lack phosphatase inhibitors."
Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.
A significant advance in sample preparation for biopharmaceutical and protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.
Features:
Easy and simple
Reliable results
Manufacturer Thermo Fisher Scientific | Available Worldwide
4.7 / 5.0 | 2 reviews