SeQuant® ZIC®-HILIC HPLC Columns by Merck KGaA, Darmstadt, Germany
Status:
Reviewer
Member since: 2017
Organization: Comenius University in Bratislava
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- Ease of use
- After sales service
- Value for money
Great and unique column.
Application Area: Analysis of drug metabolites in RBC lysates
"Great column, great and unique separation. And if you work with MS it offers a gain in sensitivity in comparison with RP columns. A bit of a downside is short column life. And you have to be careful with purity of things you put on these columns because they are prone to clogging."
Review date: 21 Jun 2017 | SeQuant® ZIC®-HILIC HPLC Columns
Status:
Advanced Reviewer Member since: 2016
Organization: Mitchell Cancer institute
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- Ease of use
- After sales service
- Value for money
Great product, marginal support
Application Area:Glycopeptides
"This column is very stable and works great for the HILIC analysis of glycopeptides. Its pressure is very stable. The sequent group however is not very friendly during tech support."
Review date: 11 Jul 2016 | SeQuant® ZIC®-HILIC HPLC Columns
Status:
Advanced Reviewer Member since: 2005
Organization: University of Tokyo
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- Ease of use
- After sales service
- Value for money
Great results!
Application Area:Plasma analysis
"ZIC-HILIC has good separation property for small polar compounds."
Review date: 28 Jun 2016 | SeQuant® ZIC®-HILIC HPLC Columns
Status:
Reviewer Member since: 2013
Organization: Idexx Labs
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- Ease of use
- After sales service
- Value for money
Great Column to have and work with...
Application Area:Analyze metabolites from plasma
"SeQuant ZIC-HILIC columns have wide range of pH. I have used it with ease and the results are highly reproducible. I feel that the price can be lowered. "
Review date: 24 Nov 2015 | SeQuant® ZIC®-HILIC HPLC Columns
Status:
Reviewer Member since: 2013
Organization: Department of Analytical Chemistry, University of Valencia
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- Ease of use
- After sales service
- Value for money
Application Area:Analytical Chemistry
"The column gave good results as expected. The quality of the packed material is really good. The worst thing is the external surface of the container because its made of plastic and it could swell."
Review date: 21 May 2014 | SeQuant® ZIC®-HILIC HPLC Columns
Status:
Reviewer Member since: 2011
Organization: Gedeon Richter Ltd
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- Ease of use
- After sales service
- Value for money
"Solution for highly polar compounds."
Review date: 05 Aug 2011 | SeQuant® ZIC®-HILIC HPLC Columns
Status:
Reviewer Member since: 2007
Organization: USM
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- Ease of use
- After sales service
- Value for money
Review date: 01 Dec 2009 | SeQuant® ZIC®-HILIC HPLC Columns
ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds.
What is HILIC?
HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents.
In HILIC one has:
- A column with a hydrophilic stationary phase
- An eluent with water, buffer and a high concentration of water-miscible organic solvent
Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC column.
The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions.
- Improved separation of hydrophilic and polar compounds
- Orthogonal selectivity to Reversed Phase (RP HPLC)
- Optimal combination with Mass Spectroscopy (MS)
- Excellent stability
Product Overview
