For sample enrichment for next-generation sequencing
• NGS platform-independent target enrichment and normalization
• Enrichment strategy: LR-PCR up to 20 kb, purification, and normalization
• Bioinformatically validated human LR-PCR primers available
• Purification by efficient removal of DNA impurities up to 2 kb
• Pooling of normalized fragments for NGS libraries with even coverage
The SeqTarget system has been developed to reduce the complexity of genomic DNA samples to a manageable subset prior to sequencing. It consists of three components — a long-range PCR (LR-PCR) amplification, PCR product purification, and normalization of target genomic DNA regions — to meet next-generation sequencing (NGS) throughput requirements. This innovative system — SeqTarget Primer Select, the SeqTarget LongRange PCR Kit, and the SeqTarget Normalization Kit — provides a complete solution for sample enrichment prior to NGS.
Kit contents: For 480 preps: 5 QIAquick 96 Plates, 5 Bead Filter 96 Plates, Normalization Beads, Buffers, and Mineral Oil. Benefits: Easy ordering of predesigned primers via the GeneGlobe Web portal.