SageELF Electrophoretic Lateral Fractionator by Sage Science

For scientists working with DNA, the generation of 12 contiguous fractions maximizes the utility of precious samples and can be used to build multiple libraries of different insert sizes for both short-read sequencers and long-range technologies that can assess structural information.

For proteomics users, SageELF delivers 12 size fractions in SDS buffer ready for analysis or identification on a mass spectrometer.

The system uses a two-dimensional process to separate DNA through an agarose column in a first direction, and then move the separated DNA fragments sideways into a linear array of buffer-filled elution modules that are positioned alongside the separation column. Each disposable cassette will process a single sample, and the instrument will process two cassettes per run.

The instrument can operate in direct current mode for samples up to mid-single kilobases in size, or in pulsed field mode for samples up to 50 kilobases in size. Fractionation range is controlled by selection of voltage protocol, gel concentration, and run timing. Internal standards can be used to improve run-to-run reproducibility, and the instrument can read fluorescein-labeled markers.