rProtein A Sepharose 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodiesIt is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage.The specificity of the recombinant protein A... Read more
rProtein A Sepharose Fast Flow
rProtein A Sepharose 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodies
It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage.
The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.
rProtein A Sepharose Fast Flow Features
•High dynamic binding capacity due to oriented, single-point attachment of protein A
•Well-established Protein A medium used in many approved MAb processes
•No mammalian components involved during the manufacturing process
It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage.
The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.
rProtein A Sepharose Fast Flow Features
•High dynamic binding capacity due to oriented, single-point attachment of protein A
•Well-established Protein A medium used in many approved MAb processes
•No mammalian components involved during the manufacturing process
Product Overview
