Agilent Routine PCR Enzymes



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Exo- Pfu DNA Polymerase
Paq5000 DNA Polymerases & PCR Master Mixes
SureStart Taq DNA Polymerase
Taq2000 DNA Polymerase
TaqPlus Precision PCR System

Exo- Pfu DNA Polymerase
Exo- Pfu DNA polymerase is a modified version of our cloned Pfu DNA polymerase suitable for [35S]dATP cycle sequencing reactions and nucleotide analog incorporation.

  • Genetically engineered mutant of cloned Pfu DNA polymerase
  • No detectable 3' to 5' exonuclease proofreading activity
  • Incorporates [35S]dATP 10 times more efficiently than Taq DNA Polymerase

Paq5000 DNA Polymerases & PCR Master Mixes
The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.

  • Robust, cost-effective alternative to Taq
  • Equal to better yield with faster cycling conditions
  • Amplify up to 6 kb genomic DNA targets
  • Easily substituted into standard PCR protocols
  • Hotstart and hotstart master mix formats available for added convenience (See Related Products)

Paq5000 DNA Polymerases & PCR Master Mixes
The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.

  • Robust, cost-effective alternative to Taq
  • Equal to better yield with faster cycling conditions
  • Amplify up to 6 kb genomic DNA targets
  • Easily substituted into standard PCR protocols
  • Hotstart and hotstart master mix formats available for added convenience (See Related Products)

SureStart Taq DNA Polymerase
SureStart Taq DNA polymerase is a hot start Taq DNA polymerase. SureStart Taq can be incorporated into PCR protocols previously optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions.

  • Hot start formulation of Taq DNA polymerase
  • Greater PCR specificity
  • Reduce nonspecific background
  • Improved product yields
  • Versatile - can be used for slow or fast activation
  • Reliable room-temperature setup
  • Use in existing PCR protocols

Taq2000 DNA Polymerase
Taq2000 DNA polymerase is a highly purified, recombinant Taq DNA polymerase cloned from the thermophilic eubacteria, Thermus aquaticus.

  • Ultrapure cloned Taq DNA polymerase
  • Virtually eliminates background artifacts
  • Most thermostable Taq DNA polymerase

ScienceTaqPlus Precision PCR System
The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

  • 2.7 times more accurate than Taq DNA polymerase
  • Optimized blend of Pfu and Taq2000 DNA polymerases
  • Amplifies templates up to 15 kb

Product Overview

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Agilent Routine PCR Enzymes

Manufacturer Agilent Technologies

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