Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites:
Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}.
Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group.
The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent derivatization and degradation during the synthesis cycle.
Standard RNA... Read more
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RNA Phosphoramidites
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Phosphoramidites for RNA oligonucleotides synthesis and more.
Features of RNA Phosphoramidites:
- Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}.
- Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group.
- The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent derivatization and degradation during the synthesis cycle.
- Standard RNA amidites provide excellent coupling efficiency when used together with either ETT or BTT as an activator.
- The purities of manufactured nucleoside phosphoramidites are quality control-checked by RP HPLC/31P-NMR analysis. (RP- HPLC: ≥ 97%, 31P-NMR: ≥ 98%)
Technical Information for RNA Phosphoramidites:
- rA(Bz)-CE phosphoramidite
- rC(Bz)-CE phosphoramidite
- rG(Ib)-CE phosphoramidite
- rU-CE phosphoramidite
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