Standard siRNA libraries require multiple aliquoting steps that can introduce variability and contamination in the absence of appropriate handling systems. Thermo Scientific Reverse Transfection Format (RTF) SMARTpool siRNA Libraries are ideal for the lab that wants to screen with siRNA, but doesn’t have access to high throughput automation.
RTF siRNA libraries are single-use libraries provided in replicate 96-well cell culture plates. SMARTpool® siRNA and validated control reagents are pre-dispensed at assay-ready amounts. No special handling is required prior to use. Simply resuspend the siRNA and add your cells.
Ensure the success of your screens with RTF Optimization Plates:
Determining appropriate experimental conditions is a critical component to successful siRNA screens. RTF Optimization plates are triplicate plates provided with single-use amounts of siRNA controls to enable the identification of optimal transfection reagent amount, cell density, and identification of the best non-targeting control for your screening assay.