Recombinant Human IGF-I/IGF-1, CF

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Recombinant Human IGF-I/IGF-1, CF
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R&D Systems Recombinant Human IGF-I/IGF-1, CF Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides. These isoforms are differentially expressed by various tissues. Specifications and Use of R&D Recombinant Human IGF-1 / ICF-1, CFSource of R&D IGF-1• A DNA sequence encoding the mature IGF-I protein (Humbel, R.E. 1984, Jin Li CH (ed.) Hormonal proteins and peptides, vol. 12, Academic Press, NY, p.57) was expressed in E. coli. Molecular Mass• The purified recombinant protein contains 70 amino acid residues and has a predicted molecular mass of approximately 7.5 kDa. Purity• >...Read more

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R&D Systems Recombinant Human IGF-I/IGF-1, CF

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides. These isoforms are differentially expressed by various tissues.

Specifications and Use of R&D Recombinant Human IGF-1 / ICF-1, CF

Source of R&D IGF-1
• A DNA sequence encoding the mature IGF-I protein (Humbel, R.E. 1984, Jin Li CH (ed.) Hormonal proteins and peptides, vol. 12, Academic Press, NY, p.57) was expressed in E. coli.

Molecular Mass
• The purified recombinant protein contains 70 amino acid residues and has a predicted molecular mass of approximately 7.5 kDa.

Purity
• > 97%, as determined by SDS-PAGE and visualized by silver stain. 

Endotoxin Level 
• < 1.0 EU per 1 μg of the cytokine as determined by the LAL method.

Activity of R&D IGF-1
• Measured in a serum-free cell proliferation assay using the human cell line MCF-7 (Karey, K.P. et al., Cancer Research, 1988, 48:4083).
• The ED50 for this effect is typically 0.3 - 1.5 ng/mL.

Formulation
• Lyophilized from a 0.2 μm filtered solution in PBS.

Reconstitution of R&D IGF-1
• It is recommended that sterile phosphate-buffered saline be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

Storage of R&D IGF-1
• Lyophilized samples are stable for up to twelve months at -20° C to -70° C.
Storage
• Upon reconstitution, this cytokine can be stored under sterile conditions at 2° - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity.
• Avoid repeated freeze-thaw cycles.

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Recombinant Human IGF-I/IGF-1, CF

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