RapiGest™ SF Protein Solubilization Reagent by Waters

"Good product. More expensive than other detergents, but it was very easy to use and did not interfere with the LC-MS analysis."

"Great results seen so far! Column has bee easy to use and effective for our purposes."

"RapiGest SF is very easy to use for the application, and easily removed (or clean-up) from the system, this makes the procedure simple and more efficient for HPLC analysis afterwards . As far as for my application, the assay reproducibility is very satisfactory."

"I am the purchaser of this product for our lab and have received positive feedback from lab members on its ease of use and reproducibility."

"RapiGestSF is so far the best solubilizing agent for most of the applications in glyco- and proteomics. The only drawback is that it's concentration needs to be high, which is decreasing the value for money. For regular day to day proteomic analyses there are other comparable reagents on the market that are used in 10x or even less final concentration."

"I have used this product for years and like it as it can easily resolubilize my protein of interest after precipitation and is a very good alternative to urea."

"My lab required a denaturant compatible to downstream MALDI-TOF applications where typical detergents such as SDS weren't feasible. RapiGest SF worked well and provided us with good results."

"The RapiGest SF reagent is fast, effective, and comprehensive. I've found it to work on a variety of antibodies and antigens; requiring no changes to the method or use of the reagent itself. The results are repetitive and precise. We are going to expand it's use outside of Glycan analysis and apply it to a number of assays that have proven problematic with a number of our proprietary proteins."

"I love to use Rapigest for any in solution digestion, from FFPE to cell lysate."

"We use RapiGest in the denaturation step to prepare proteomics and peptide mapping samples. it is easy to use. We acquired high quality data. We didn't have a lot of experience on after sales care, since we didn't encounter any issue with the product."

"This is a great substitute of other ionic detergents, such as SDS, that are not amenable for mass spectrometry. For my applications, the solubilization effectiveness was almost as good as SDS and its removal by acid treatment was excellent. This product is very helpful, especially when your sample is limiting and you need high sensitivity. I find it somewhat expensive, especially if you need to scale up, but is is worth the cost."

"Easy to use as the instructions are thorough making the product paramount for protein isolation."

"The RapiGest reagent worked great for the protein work that I am currently doing. it got the protein in solution so I could digest it and run it on our triple TOF mass spec. Cost was relatively low for the product and would definitely purchase again."

"I obtained very consistent results while using this product"

"This product is very helpful, we can denature the protein more efficiently and use less enzyme to release the N-glycan."

"It is helpful for protein deglycosylation and intact mass analysis. Since it is mass spec compatible, the solution can be directly injected to mass spec for intact mass analysis. This is especially useful when protein denature is required to remove some N-link glycans."

"The product provides excellent reproducibliity for generating proteolytic digests from monoclonal antibody."

"Ease of use and improved results combined with an affordable price make this item a 5-star winner!"

"Great value and ease of use. The prep time is only 30 minutes."

"This product has been extremely helpful in the extraction of proteins from my neuron samples. Using RapiGest, I am able to digest the protein immediately after extraction without buffer exchange. RapiGest precipitates nicely out of solution prior to desalting the peptides and does not interfere with downstream mass spectrometry analysis. I would highly recommend this product to anyone performing tissue lysis prior to mass spectrometry analysis."

"Tried it and found the results were good, but company wanted us to stick to the current sample preparation method. We therefore don't have too much experience with this product."

"Great product. Easy to use and easy to edit protocol to fit other needs. Great end results."

"Straightforward to use and gives good reproducible mass spectrometry measurement. It would be nice to have it for lower price since sometimes we have to upscale proteins samples and it becomes expensive."

"Yes, we believe Rapigest helped in our protein isolation and proteomics analysis. The only thing what we noticed is amount of protein isolated was low compared other protein isolation methods."

"Very good surfactant especially on the protein level. However, if used for peptide digestion causes significant non-specific cleavages."

"It increased the efficiency of enzyme digestion or reduction of the antibodies. The instruction is very simple and clear to understand."

"The product allows me to obtain reproducible results at high quality. The most important benefit of this product is that it allows me to analyze samples with various degrees of contamination from RNA. It is a great fit for projects where the classical proteomics homogenization/digestion approaches fail. Provides a greater recovery from less soluble peptides."

"It has characterized items well with right quantities of digestion enzyme. Easy to use. "